Development of inter-family nuclear transplant embryos by transplanting the nuclei from the loach blastulae into the non-enucleated zebrafish eggs

Li Li; Zhang Shicui; Yuan Jinduo; Li Hongyan

doi:10.1007/BF02842762

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Development of inter-family nuclear transplant embryos by transplanting the nuclei from the loach blastulae into the non-enucleated zebrafish eggs

OriginalPaper | Updated：2023-05-16

- Development of inter-family nuclear transplant embryos by transplanting the nuclei from the loach blastulae into the non-enucleated zebrafish eggs
- Development of inter-family nuclear transplant embryos by transplanting the nuclei from the loach blastulae into the non-enucleated zebrafish eggs
- 海洋湖沼学报（英文） 2003年21卷第1期页码：64-71
- Affiliations：
  
  1. Department of Marine Biology Ocean University of Qingdao
  2. Department of Biotechnology Shenzhen University
- Author bio：
- Funds：
  
  Project supported by the Ministry of Science and Technology (863-819-04-06).
- DOI：10.1007/BF02842762
  中图分类号：
- 纸质出版：2003
- Accepted：
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Development of inter-family nuclear transplant embryos by transplanting the nuclei from the loach blastulae into the non-enucleated zebrafish eggs[J]. 海洋湖沼学报（英文）, 2003,21(1):64-71.

Li, L., Shicui, Z., Jinduo, Y. et al. Development of inter-family nuclear transplant embryos by transplanting the nuclei from the loach blastulae into the non-enucleated zebrafish eggs., Chin. J. Ocean. Limnol. 21, 64–71 (2003). https://doi.org/10.1007/BF02842762
Development of inter-family nuclear transplant embryos by transplanting the nuclei from the loach blastulae into the non-enucleated zebrafish eggs[J]. 海洋湖沼学报（英文）, 2003,21(1):64-71. DOI： 10.1007/BF02842762.

Li, L., Shicui, Z., Jinduo, Y. et al. Development of inter-family nuclear transplant embryos by transplanting the nuclei from the loach blastulae into the non-enucleated zebrafish eggs., Chin. J. Ocean. Limnol. 21, 64–71 (2003). https://doi.org/10.1007/BF02842762 DOI：

摘要

Abstract

The developmental fate of the pronuclei in recombined embryos obtained by transplanting the donor nuclei into the non-enucleated eggs remains controversial in the case of fish. In the present study

the nuclei from the loach blastulae were transplanted into non-enucleated zebrafish eggs

the resulting 9 inter-family nuclear transplant embryos developed to larval stages. Although the development timing of the nuclear transplants resembled that of zebrafish

chromosome examination revealed that most of the recombined embryos were diploids with karyotype characteristic of loach

which was also proved by RAPD analysis. Moreover

3 out of the 9 larval fish formed barb rudiments specific to loach. It was therefore concluded that the nuclear transplant larval fish were inter-family nucleo-cytoplasmic hybrids; and that only the donor nuclei were involved in the development of the nuclear transplant embryos

while the pronuclei in the non-enucleated eggs were likely automatically eliminated during the development.

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references

Gasaryan, K. G., Hung, N. M., Neyfakh, A. A. et al., 1979. Nuclear transplantation in teleost Misgurnus fossilis L.Nature 280 (16): 585–587.

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Yi, M. S., Yu, Q. X., Zhou, R. J., 1997. The karyotype and Ag-NORs, C-banding patterns of the zebrafish (Brachydanio rerio).Hereditas. 19 (suppl.): 59–60. (in Chinese with English abstract)

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Yu, X. J., Zhou, T., Li, Y. C. et al., 1989. Chromosomes of Chinese fresh-water fishes, Science Press, Beijing, p. 104–143. (in Chinese)

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