Induction of ectodermal cells from vegetal-endodermal blastomeres of amphioxus (Branchiostoma belcheri tsingtaunese) embryos by the calcium ionophore A23187

Zhang Shi-cui; Zheng Jia-sheng; Zhang Hong-wei; Mao Bing-yu

doi:10.1007/BF02842706

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Induction of ectodermal cells from vegetal-endodermal blastomeres of amphioxus (Branchiostoma belcheri tsingtaunese) embryos by the calcium ionophore A23187

OriginalPaper | Updated：2023-05-16

- Induction of ectodermal cells from vegetal-endodermal blastomeres of amphioxus (Branchiostoma belcheri tsingtaunese) embryos by the calcium ionophore A23187
- Induction of ectodermal cells from vegetal-endodermal blastomeres of amphioxus (Branchiostoma belcheri tsingtaunese) embryos by the calcium ionophore A23187
- 海洋湖沼学报（英文） 1999年17卷第2期页码：97-104
- Affiliations：
  
  1. College of Marine Life Sciences Ocean University of Qingdao
  2. Department of Biology Shandong University
- Author bio：
- Funds：
  
  Project 39470091 supported by NSFC and partly supported by Shandong Natural Science Foundation, Grant No.93D0140.
- DOI：10.1007/BF02842706
  中图分类号：
- 纸质出版：1999
- Accepted：
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Induction of ectodermal cells from vegetal-endodermal blastomeres of amphioxus (Branchiostoma belcheri tsingtaunese) embryos by the calcium ionophore A23187[J]. 海洋湖沼学报（英文）, 1999,17(2):97-104.

Shi-cui, Z., Jia-sheng, Z., Hong-wei, Z. et al. Induction of ectodermal cells from vegetal-endodermal blastomeres of amphioxus (Branchiostoma belcheri tsingtaunese) embryos by the calcium ionophore A23187., Chin. J. Ocean. Limnol. 17, 97–104 (1999). https://doi.org/10.1007/BF02842706
Induction of ectodermal cells from vegetal-endodermal blastomeres of amphioxus (Branchiostoma belcheri tsingtaunese) embryos by the calcium ionophore A23187[J]. 海洋湖沼学报（英文）, 1999,17(2):97-104. DOI： 10.1007/BF02842706.

Shi-cui, Z., Jia-sheng, Z., Hong-wei, Z. et al. Induction of ectodermal cells from vegetal-endodermal blastomeres of amphioxus (Branchiostoma belcheri tsingtaunese) embryos by the calcium ionophore A23187., Chin. J. Ocean. Limnol. 17, 97–104 (1999). https://doi.org/10.1007/BF02842706 DOI：

摘要

Abstract

Timing of vegetal-endodermal cell determination in amphioxus embryos remains uncertain. We tentatively tested effects of A23187

the calcium ionophore

on the development of vegetal blastomeres isolated at the 16-cell stage. It was found that when vegetal blastomeres committed to endoderm were treated with A23187 prior to gastrulation

they were transformed into ectodermal cells as evidenced by the cell morphology and function characteristic of epidermis. However

the developmental fate of the same blastomeres untreated or treated with DMSO at the same stage or of those treated with A23187 after gastrulation remained unchanged. Thus

vegetal-endodermal cells in amphioxus embryos are not irreversibly determined before the gastrula stage

and artificial increase in intracelluar Ca2+ concentration can induce transdetermination of the predetermined endodermal cells into ectodermal cells.

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references

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