The genotoxic effects of benzo[a]pyrene and methamidophos on black porgy evaluated by comet assay

Liu Rixian; Hong Huasheng; Wang Xinhong; Wang Kejian; Wang Chunguang

doi:10.1007/BF02842691

Your Location：

Home >

Browse articles >

The genotoxic effects of benzo[a]pyrene and methamidophos on black porgy evaluated by comet assay

OriginalPaper | Updated：2023-05-16

- The genotoxic effects of benzo[a]pyrene and methamidophos on black porgy evaluated by comet assay
- The genotoxic effects of benzo[a]pyrene and methamidophos on black porgy evaluated by comet assay
- 海洋湖沼学报（英文） 2005年23卷第4期页码：455-460
- Affiliations：
  
  State Key Laboratory for Marine Environmental Science Environmental Science Research Center Xiamen University
- Author bio：
- Funds：
  
  The work was supported by Natural Science Foundation of China (Grant No. A20077023 and C40106012)
- DOI：10.1007/BF02842691
  中图分类号：
- 纸质出版：2005
- Accepted：
Scan QR Code
The genotoxic effects of benzo[a]pyrene and methamidophos on black porgy evaluated by comet assay[J]. 海洋湖沼学报（英文）, 2005,23(4):455-460.

Rixian, L., Huasheng, H., Xinhong, W. et al. The genotoxic effects of benzo[a]pyrene and methamidophos on black porgy evaluated by comet assay., Chin. J. Ocean. Limnol. 23, 455–460 (2005). https://doi.org/10.1007/BF02842691
The genotoxic effects of benzo[a]pyrene and methamidophos on black porgy evaluated by comet assay[J]. 海洋湖沼学报（英文）, 2005,23(4):455-460. DOI： 10.1007/BF02842691.

Rixian, L., Huasheng, H., Xinhong, W. et al. The genotoxic effects of benzo[a]pyrene and methamidophos on black porgy evaluated by comet assay., Chin. J. Ocean. Limnol. 23, 455–460 (2005). https://doi.org/10.1007/BF02842691 DOI：

摘要

Abstract

In this study

two common pollutants (benzo[a]pyrene and methamidophos) in marine environment were tested by comet assay for their inducement ofin vivo genotoxic effect to the blood cells of black porgy (Acanthopagrus schlegeli). The fish was exposed to 2 μg/L of benzo[a]pyrene (BaP) and methamidophos

and their mixture. The assay was performed on whole blood at 2 h

5 h

24 h and 96 h exposure intervals. A significant increase in DNA damage was observed in each treatment with the pollutants. Additive effect of BaP and methamidophos was also found in the experiment. However

the decrease ratios of DNA damage for 5 h and 96 h exposure interals compared with 2 h and 24 h exposure ones

respectively

were noticed. This phenomenon may be explained by the function of repairing process via enzyme cytochrome P450 in the animal. Evidence of the genotoxicity of organophosphorus pesticides (OPs) and polynuclear aromatic hydrocarbons (PAHs) on marine fish are discussed in this paper.

关键词

Keywords

references

Anderson, D. T. W. Yu and B. J. Phillips, 1994. The effect of various antioxidants and other modifying agents on oxygen-radical-generated DNA damage in human lymphocytes in the COMET assay.Mut. Res. 307: 261–271.

Bailey, G. S., D. E. Williams and J. D. Hendricks, 1996. Fish models for environmental carcinogenesis in the rainbow trout.Environ. Health Perspect. 104(S1): 5–21.

Banu, B. S., K. Danadevi and M. F. Rahman, 2001. Genotoxic effect of monocrotophos to sentinel species using comet assay.Food Chem. Toxicol. 39: 361–366.

Bartzatt, R., Q. Huang and D. Nagel, 1991. Cytochrome P-450 IIBI, dealkylates nitrosamine induced adducts.Med. Sci. Res. 19: 517–518.

Blasiak, J., P. Jaloszynski and A. Trzeciak, 1999. In vitro studies on the genotoxicity of the organophosphorus insecticide malathion and its two analogues.Mut. Res. 445: 275–283.

Collins, A. R., M. Ai-guo and S. J. Duhie, 1995. The kinetics of repair of oxidative DNA damage (strand breaks and oxidised pyrimidines) in human cells.Mut. Res. 336: 69–77.

DeMarco, A., R. DeSalvia and S. Polani, 2000. Evaluation of genotoxic and cytotoxic properties of pesticides employed in Italian agricultural practices.Environ. Res. 83: 311–321.

Devaux, A., P. Flammarion and V. Bernardon, 1998. Monitoring of the Chemical Pollution of the River Rhône through Measurement of DNA Damage and Cytochrome P4501A Induction in Chub (Leuciscus cephalus).Mar. Env. Res. 46(1–5): 257–262.

Godard, T., V. Fessard, S. Huet et al., 1999. Comparativein vitro andin vivo assessment of genotoxic effects of etoposide and chlorothalonil by the comet assay.Mut. Res. 444: 103–116.

Helma, C. and M. Uhl., 2000. A public domain image-analysis program for the single-cell gel-electrophoresis (comet) assay.Mut. Res. 466: 9–15.

Maria, V. L., A. C. Correia and M. A. Santos, 2002. Anguilla anguilla L. biochemical and genotoxic responses to benzo[a]pyrene.Ecotox. and Env. Safety 53(1): 86–92.

Maskaoui, K., J. L. Zhou, H. S. Hong et al., 2002. Contamination by polycyclic aromatic hydrocarbons in the Jiulong River Estuary and Western Xiamen Sea, China.Env. Pollut. 118: 109–122.

Mitchelmore, C. L. and J. K. Chipman, 1998. DNA strand breakage in aquatic organisms and the potential value of the comet assay in environmental monitoring.Mut. Res. 399: 135–147.

Rahman, M. F., M. Mahboob, K. Danadevi et al., 2002. Assessment of genotoxic effects of chloropyriphos and acephate by the comet assay in mice leucocytes.Mut. Res. 516: 139–147.

Salmenova, D., A. Gabelova, L. Ruzekova et al., 1997. Validations of comet assay against DNA unwinding technique, alkaline elution of DNA and chromosomal aberrations.Mut. Res. 383: 243–252.

Singh, N. P., M. T. McCoy, R. R. Tice et al., 1988. A simple technique for quantitation of low levels of DNA damage in individual cells, Exp.Cell Res. 175: 184–191.

Uhl, M., C. Helma and S. Knasmuller, 1999. Single-cell gel electrophoresis assays with human-derived hepatoma (HepG2) cells.Mut. Res. 441: 215–224.

Vigreux, C., J. M. Poul, E. Deslandes et al., 1998. DNA damaging effects of pesticides measured by the single cell gel electrophoresis assay (comet assay) and the chromosomal aberration test in CHOKI cells.Mut. Res. 419: 79–90.

Villani, P., C. Andreoli, R. Crebelli et al., 1998. Analysis of micronuclei and DNA single strand breaks in mouse splenocytes and peripheral lymphocytes after oral administration of tetramethylthiuram disulfide (Thiram),Food Chem. Toxicol. 36: 155–164.

Villarini, M., M. Moretti, R. Pasquini et al., 1998. In vitro genotoxic effects of the insecticide deltamethrin in human peripheral blood leucocytes: DNA damage (comet assay) in relation to the induction of sister chromatid exchanges and micronuclei.Toxicology 130: 129–139.

Villarini, M., M. Moretti, E. Damiani et al., 1998. Detection of DNA damage in stressed trout nucleated erythrocytes using the comet assay: protection by nitroxide radicals.Free Rad. Bio. Med. 24(7–8): 1310–1315.

Vincent, B., A. W. Dennis, G. Emma et al., 2001. Application of the comet and micronucleus assays to butterfish (Pholis gunnellus) erythrocytes from the Firth of Forth, Scotland.Chemosphere 44(3): 383–392.

Wild, D., 1975. Mutagenicity studies on organophosphorus insecticides.Mutat. Res. 32: 133–150.

Zhang, Z. L., H. S. Hong and X. H. Wang, 2002. Determination and load of organophosphorus and organochlorine pesticides at water from Jiulong River Estuary, China.Mar. Pollut. Bull. 45: 397–402.

Zhang, Z. L., M. H. Dai, H. S. Hong et al., 2002. Dissolved insecticides and polychlorinated biphenyls in the Pearl River Estuary and South China Sea.J. Environ. Monit. 4: 922–928.

Zhong, Y., S. L. Feng, Q. Su et al., 2000. Study on monitoring genotoxicant pollution in surface water of Yang Zhou city using micronucleus test and comet assay. Carcino.Terato. and Mut. 12(1): 18–23.

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Effects of ration, temperature and body weight on metabolism in young black porgy

Using DNA damage to monitor water environment

Roles of polysaccharide from Branchiostoma belcheri in anti-DNA oxidation and anti-tumor activity in S180 mice

Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure

Effect of pollution on DNA damage and essential fatty acid profile in Cirrhinus mrigala from River Chenab