Cultural characteristics of chromium resistant unicellular cyanobacteria isolated from local environment in Pakistan[J]. 海洋湖沼学报(英文), 2005,23(4):433-441.
Hameed, A., Hasnain, S. Cultural characteristics of chromium resistant unicellular cyanobacteria isolated from local environment in Pakistan., Chin. J. Ocean. Limnol. 23, 433–441 (2005). https://doi.org/10.1007/BF02842688
Cultural characteristics of chromium resistant unicellular cyanobacteria isolated from local environment in Pakistan[J]. 海洋湖沼学报(英文), 2005,23(4):433-441. DOI: 10.1007/BF02842688.
Hameed, A., Hasnain, S. Cultural characteristics of chromium resistant unicellular cyanobacteria isolated from local environment in Pakistan., Chin. J. Ocean. Limnol. 23, 433–441 (2005). https://doi.org/10.1007/BF02842688DOI:
Cultural characteristics of chromium resistant unicellular cyanobacteria isolated from local environment in Pakistan
摘要
Abstract
Many unicellular cyanobacteria were isolated from different places: fields
ponds
polluted water
and soils from Muredkey and Kasur tannery areas
near Lahore
Pakistan. Different media like BG 11 medium
Bold Basal medium
Chu's #10 medium and Gorham's medium
in standard forms and with slight variations of ingredients
and different pH
temperature and light regimes were checked for the optimum growth of the isolates. The isolation procedure was repeated with different concentrations of chromium to select the resistant strains. These selected strains grew on chromium of the range 100–200 μg/ml in BG 11 medium. Cyanobacteria were maintained in solid and liquid media with/without shaking. Cyanobacterial strains were collected from natural habitats that were accompanied by a diversified group of organisms including bacteria
protozoan
and rotifers etc. In order to eliminate these agents termed as contaminants
we used several methods including phenol treatment
use of antibiotic and careful manual picking of unicellular cyanobacteria. Resistance of these strains against different heavy metals (ZnSO4
MnSO4
NiSO4
CoCl2
Pb(NO3)3
CuSO4
HgCl2
AgNO3 and CdCl2) and antibiotics (erythromycin
streptomycin
kanamyci chloramphenicol
neomycin) was evaluated. Optimum temperature was 30°C with variable pH for the reduction of Cr6+ in to Cr3+ in majority of strains.
关键词
Keywords
references
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相关作者
Lingling WAN
Chunlei SONG
Yiyong ZHOU
Xiuyun CAO
Qiang HE
Yiyao WANG
Ming LI
陈义中
相关机构
Key Laboratory of Algal Biology, State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences
College of Natural Resources and Environment, Northwest A&F University, Yangling
Chengdu Environmental Protection Research Institute
State Key Laboratory of Estuarine and Coastal Research, East China Normal University