Method for observation of deembedded sections of fish gonad by scanning electron microscopy

Mao Lian-ju

doi:10.1007/BF02842677

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Method for observation of deembedded sections of fish gonad by scanning electron microscopy

OriginalPaper | Updated：2023-05-16

- Method for observation of deembedded sections of fish gonad by scanning electron microscopy
- Method for observation of deembedded sections of fish gonad by scanning electron microscopy
- 海洋湖沼学报（英文） 2000年18卷第3期页码：283-288
- Affiliations：
  
  Department of Aquaculture Dalian Fisheries College
- Author bio：
- Funds：
- DOI：10.1007/BF02842677
  中图分类号：
- 纸质出版：2000
- Accepted：
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Method for observation of deembedded sections of fish gonad by scanning electron microscopy[J]. 海洋湖沼学报（英文）, 2000,18(3):283-288.

Lian-ju, M. Method for observation of deembedded sections of fish gonad by scanning electron microscopy., Chin. J. Ocean. Limnol. 18, 283–288 (2000). https://doi.org/10.1007/BF02842677
Method for observation of deembedded sections of fish gonad by scanning electron microscopy[J]. 海洋湖沼学报（英文）, 2000,18(3):283-288. DOI： 10.1007/BF02842677.

Lian-ju, M. Method for observation of deembedded sections of fish gonad by scanning electron microscopy., Chin. J. Ocean. Limnol. 18, 283–288 (2000). https://doi.org/10.1007/BF02842677 DOI：

摘要

Abstract

This article reports a method for examining the intracellular structure of fish gonads using a scanning electron microscope(SEM). The specimen preparation procedure is similar to that for transmission electron microscopy wherein samples cut into semi-thin sections are fixed and embedded in plastic. The embedment matrix was removed by solvents. Risen-free specimens could be observed by SEM. The morphology of matured sperms in the gonad was very clear

and the oocyte internal structures appeared in three-dimensional images. Spheroidal nucleoli and yolk vesicles and several bundles of filaments adhered on the nucleoli could be viewed by SEM for the first time.

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references

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