Detection of white spot syndrome virus (WSSV) ofPenaeus chinensis by in situ hybridization

Zhan Wen-bin; Wang Yuan-hong; Zhang Zhi-dong; Fukuda Hideo

doi:10.1007/BF02842670

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Detection of white spot syndrome virus (WSSV) ofPenaeus chinensis by in situ hybridization

OriginalPaper | Updated：2023-05-16

- Detection of white spot syndrome virus (WSSV) ofPenaeus chinensis by in situ hybridization
- Detection of white spot syndrome virus (WSSV) ofPenaeus chinensis by in situ hybridization
- 海洋湖沼学报（英文） 2000年18卷第3期页码：241-246
- Affiliations：
  
  1. Aquaculture Research Laboratory Ocean University of Qingdao
  2. Tokyo University of Fisheries
- Author bio：
- Funds：
  
  This work was supported by SRF for ROCS, SEM and the National 863 Project (Grant 819-Q-08) and Project under major State Basic Research Development Program (Grant G1999012002).
- DOI：10.1007/BF02842670
  中图分类号：
- 纸质出版：2000
- Accepted：
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Detection of white spot syndrome virus (WSSV) ofPenaeus chinensis by in situ hybridization[J]. 海洋湖沼学报（英文）, 2000,18(3):241-246.

Wen-bin, Z., Yuan-hong, W., Zhi-dong, Z. et al. Detection of white spot syndrome virus (WSSV) ofPenaeus chinensis by in situ hybridization., Chin. J. Ocean. Limnol. 18, 241–246 (2000). https://doi.org/10.1007/BF02842670
Detection of white spot syndrome virus (WSSV) ofPenaeus chinensis by in situ hybridization[J]. 海洋湖沼学报（英文）, 2000,18(3):241-246. DOI： 10.1007/BF02842670.

Wen-bin, Z., Yuan-hong, W., Zhi-dong, Z. et al. Detection of white spot syndrome virus (WSSV) ofPenaeus chinensis by in situ hybridization., Chin. J. Ocean. Limnol. 18, 241–246 (2000). https://doi.org/10.1007/BF02842670 DOI：

摘要

Abstract

White Spot Syndrome Virus (WSSV) was purified from hemolymph of infected shrimp. After nucleic acid extraction from the purified virus particles

EcoR I-digested fragments of the WSSV genome were cloned; three of these fragments were used as non-radioactive probes labeled with DIG-11-dUTP. The probes hybridized in situ

with sections located in the nuclei of all WSSV-infected tissues. The virus was detected in the gill

stomach

epidermis

and connective tissue and so on

but not detected in healthy shrimp tissues and epithelial cells of hepatopancreatic tubules of diseased shrimp.

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references

Chang, P. S., Lo, C. F., Wang, Y. C. et al., 1996. Identification of white spot syndrome associated baculovirus (WSBV) target organs in the shrimpPenaeus monodon by in situ hybridization.Dis. Aquat. Org. 27: 131–139.

Durand, S., Lightner, D. V., Nunan, L. et al., 1996. Application of gene probes as diagnostic tools for white spot baculovirus (WSBV) of penaeid shrimp.Dis. Aquat. Org. 27: 59–66.

Kimura, T., Yamano, K., Nakano, H. et al., 1996. Detection of penaeid rod—shaped DNA virus (PRDV) by PCR.Fish Pathol. 31: 93–98.

Lo, C. F., Leu, J. H., Ho, C. H. et al., 1996. Detection of baculovirus associated with white spot syndrome (WSBV) in penaeid shrimp using polymerase chain reaction.Dis. Aquat. Org. 25: 133–141.

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Mari, J., Lightner, D. V., Poulos, B. T. et al., 1995. Partial cloning of the genome of an unusual shrimp parvovirus (HPV): use of gene probes in disease diagnosis.Dis. Aquat. Org. 22: 129–134.

Poulos, B. T., Mari, J., Bonami, J. R. et al., 1994. Use of non—radioactively labeled DNA probes for the detection of a baculovirus fromPenaeus monodon by in situ hybridization on fixed tissue.J. Virol. Meth. 49: 187–194.

Sambroock, J., Fritsch, E. F., Maniatis, T. et al., 1989. Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, p. 21–104.

Zhan, W. B., Yu, K. K., Meng, Q. X. et al., 1995. Study on baculovirus disease ofPenaeus chinensis.J. Fishery Science of China 2: 22–28.

Zhan, W. B., Wang, Y. H., Fryer, J. L. et al., 1998. White spot syndrome virus infection of cultured shrimp in China.J. Aquatic Animal Health 10: 405–410.

Zhan, W. B., Wang, Y. H., Fryer, J. L. et al., 1999. Production of monoclonal antibodies against white spot syndrome virus.J. Aquatic Animal Health 11: 17–22.

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