Comparative studies of inorganic carbon utilization inEmiliania huxleyi and some non-calcifying marine microalgae

Dong Liang-feng; M. J. Merrett; Wu Chao-yuan

doi:10.1007/BF02842599

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Comparative studies of inorganic carbon utilization inEmiliania huxleyi and some non-calcifying marine microalgae

OriginalPaper | Updated：2023-05-16

- Comparative studies of inorganic carbon utilization inEmiliania huxleyi and some non-calcifying marine microalgae
- Comparative studies of inorganic carbon utilization inEmiliania huxleyi and some non-calcifying marine microalgae
- 海洋湖沼学报（英文） 1999年17卷第3期页码：219-232
- Affiliations：
  
  1. Department of Biology John Tabor Laboratory University of Essex
  2. School of Biological Sciences University of Wales Swansea
  3. Department of Marine Botany Institute of Oceanology Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Contribution No. 3557 from the Institue of Oceanology, Chinese Academy of Sciences.;This work was supported by NERC grant GRE3/7853 U. K. and partly supported by Bio-Engineering Center, SSTC 96-C01-05-01.
- DOI：10.1007/BF02842599
  中图分类号：
- 纸质出版：1999
- Accepted：
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Comparative studies of inorganic carbon utilization inEmiliania huxleyi and some non-calcifying marine microalgae[J]. 海洋湖沼学报（英文）, 1999,17(3):219-232.

Liang-feng, D., Merrett, M.J. & Chao-yuan, W. Comparative studies of inorganic carbon utilization inEmiliania huxleyi and some non-calcifying marine microalgae., Chin. J. Ocean. Limnol. 17, 219–232 (1999). https://doi.org/10.1007/BF02842599
Comparative studies of inorganic carbon utilization inEmiliania huxleyi and some non-calcifying marine microalgae[J]. 海洋湖沼学报（英文）, 1999,17(3):219-232. DOI： 10.1007/BF02842599.

Liang-feng, D., Merrett, M.J. & Chao-yuan, W. Comparative studies of inorganic carbon utilization inEmiliania huxleyi and some non-calcifying marine microalgae., Chin. J. Ocean. Limnol. 17, 219–232 (1999). https://doi.org/10.1007/BF02842599 DOI：

摘要

Abstract

Inorganic carbon utilization in the non-calcifying marine microalgae

Nannochloropsis oculata

Phaeodactylum tricornutum andPorphyridium purpureum was compared with high- and low-calcifying strains ofEmiliania huxleyi grown in artificial seawater medium aerated with either air (0.03% V/V CO2) or CO2-free air. For high-calcifying strain ofE. oculata andP. tricornutem

similar growth patterns were observed in air-and CO2-free air-grown cultures.P. purpureum showed a less final cell density in CO2-free air than in air-grown culture. However

low-calcifying strain ofE. huxleyi was able to grow only in air-grown culture

but not in CO2-free air-grown culture. Measurements of alkalinity

concentration of dissolved inorganic carbon (DIC) and free CO2 showed different patterns of DIC utilization. WithN. oculata

P. tricornutum andP. purpureum the pattern of DIC utilization was characterized by an increase of pH and a decrease of DIC but a constant alkalinity in the cultures aerated with air or CO2-free air

suggesting that bicarbonate utilization was concomitant with an efflux of OH−. Both alkalinity and pH were maintained rather constant in air-grown culture of low-calcifying strain ofE. huxleyi

suggesting that diffusive entry of CO2 could meet the requirement of DIC for its photosynthesis and growth. High-calcifying strain ofE. huxleyi

however

showed a pattern of decrease of alkalinity and DIC but an almost constant pH

indicating that bicarbonate was the major form of inorganic carbon utilised by this organism and bicarbonate uptake is unlikely to be accompanied by an efflux of OH−. The final pH values reached byN. oculata

P. tricornutum andP. purpureum in a closed system were 10.75

10.60 and 9.85 respectively

showing that bicarbonate utilisation is concomitant with an efflux of OH−. While the final pH of 8.4 in high-calcifyingE. huxleyi suggests that bicarbonate utilization was not accompanied by an efflux of OH−.

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