Biochemical characterization of mannuronan C5-epimerase from <italic style="font-style: italic">Saccharina</italic> <italic style="font-style: italic">japonica</italic>

Xue MENG; Shuang LI; Jie ZHANG; Delin DUAN

doi:10.1007/s00343-025-5173-2

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Biochemical characterization of mannuronan C5-epimerase from Saccharina japonica

Updated：2026-01-19

- Biochemical characterization of mannuronan C5-epimerase from Saccharina japonica
- Journal of Oceanology and Limnology Pages: 1-12(2026)
- Affiliations：
  
  1.Key Lab of Breeding Biotechnology & Sustainable Aquaculture, Shandong Province Key Lab of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  2.Lab for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao 266237, China
  3.University of Chinese Academy of Sciences, Beijing 100049, China
- Author bio：
  
  dlduan@qdio.ac.cn
- Funds：
- DOI：10.1007/s00343-025-5173-2
  CLC：
- Received：26 May 2025，
  
  Online First：19 January 2026，
- Accepted：
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MENG Xue,LI Shuang,ZHANG Jie,et al.Biochemical characterization of mannuronan C5-epimerase from ,Saccharina ,japonica[J].Journal of Oceanology and Limnology,
DOI：

MENG Xue,LI Shuang,ZHANG Jie,et al.Biochemical characterization of mannuronan C5-epimerase from ,Saccharina ,japonica[J].Journal of Oceanology and Limnology, DOI：.

摘要

Abstract

Alginate is a polysaccharide predominantly synthesized by brown algae and bacteria. It is a linear polymer composed of β-D-mannuronic acid (M) and its C5 reverse isomer α-L-guluronic acid (G). Currently

there is a significant demand for high-G-content alginate in the market. Mannuronan C5-epimerase (MC5E) is one important enzyme in the alginate synthesis as it catalyzes epimerization of β-D-mannuronic acid (M) to α-L-guluronic acid (G) at the polyM polymer level. We screened and isolated one high expression

MC5E

sequence from the

Saccharina

japonica

genome database and demonstrated that

SjMC5E1

could epimerize and increase the content of G residues in alginate. Additionally

we detected the mannuronan C5-epimerase activity of

SjMC5E1

was optimal at 37 °C in the temperature range of 15–50 °C

the

was 0.30 and the M/G ratio of alginate was the lowest at 2.32. The biochemical characterization of

SjMC5E1

provides valuable insights into its role in alginate biosynthesis

particularly in the conversion of M to G. These findings contribute to a better understanding of the enzymatic mechanisms underlying alginate synthesis and may support future efforts to optimize alginate production. However

further studies are needed to fully explore the potential of

SjMC5E1

in tailoring alginates with specific properties for industrial or biomedical applications.

关键词

Keywords

references

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⁰

Biochemical characterization of mannuronan C5-epimerase from Saccharina japonica

DOI：10.1007/s00343-025-5173-2

摘要

Abstract

关键词

Keywords

references