A potential germ cell-specific marker in Japanese flounder, <italic style="font-style: italic">Paralichthys olivaceus</italic>: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)

Yang YANG; Qinghua LIU; Daoyuan MA; Zongcheng SONG; Jun LI

doi:10.1007/s00343-019-7390-z

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A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)

Biology | Updated：2023-05-19

- A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)
- Chinese Journal of Oceanology and Limnology Vol. 36, Issue 4, Pages: 1342-1348(2018)
- Affiliations：
  
  1.Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  2.Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China
  3.University of Chinese Academy of Sciences, Beijing 100049, China
  4.Weihai Shenghang Aquatic Product Science and Technology Co. Ltd., Weihai 264200, China
- Author bio：
  
  LIU Qinghua, qinghualiu@qdio.ac.cn
  LI Jun, junli@qdio.ac.cn
- Funds：
- DOI：10.1007/s00343-019-7390-z
  CLC：
- Received：19 December 2017，
  
  Accepted：22 February 2018，
  
  Online First：27 February 2017，
  
  Published：2018-07
- Accepted：
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Yang YANG, Qinghua LIU, Daoyuan MA, et al. A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(4): 1342-1348.
DOI：

Yang YANG, Qinghua LIU, Daoyuan MA, et al. A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(4): 1342-1348. DOI： 10.1007/s00343-019-7390-z.

摘要

Abstract

Some germ cell marker genes

such as

vasa

nanos

and

dead end

(

dnd

)

have been identified in fish. Recently

lymphocyte antigen 75 (Ly75/CD205) has been identified as a mitotic germ cell-specific cell-surface marker in several fish species. In this study

the Japanese flounder

Paralichthys olivaceus

ly75 homolog (

ly75

) was cloned and its expression pattern in gonads was analyzed. The full-length cDNA of

ly75

was 7 346 bp

with an open reading frame (ORF) of 5 229 bp. The ORF encoded a protein containing 1 742 amino acids with a predicted molecular mass of 196.89 kDa. In adult tissues

ly75

transcripts were detected in all analyzed tissues but abundantly in the testis. In in-situ hybridization analyses

ly75

mRNA was predominantly localized in oocytes in the ovary and spermatogonia in the testis

but

ly75

mRNA was not detected in oogonia

spermatocytes

spermatids

or spermatozoa. These results indicated that

ly75

could be a potential germ cell-specific marker in

P. olivaceus

as in other fishes.

关键词

Keywords

references

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Related Institution

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A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)

DOI：10.1007/s00343-019-7390-z

摘要

Abstract

关键词

Keywords

references