Assessment of different <italic style="font-style: italic">mcy</italic> genes for detecting the toxic to non-toxic <italic style="font-style: italic">Microcystis</italic> ratio in the field by multiplex qPCR

Jun ZUO; Liting CHEN; Kun SHAN; Lili HU; Lirong SONG; Nanqin GAN

doi:10.1007/s00343-019-7186-1

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Assessment of different mcy genes for detecting the toxic to non-toxic Microcystis ratio in the field by multiplex qPCR

ICTC-10 Special Issue: Cyanobacteria and cyanotoxins: responses and detection | Updated：2023-05-19

- Assessment of different mcy genes for detecting the toxic to non-toxic Microcystis ratio in the field by multiplex qPCR
- Chinese Journal of Oceanology and Limnology Vol. 36, Issue 4, Pages: 1132-1144(2018)
- Affiliations：
  
  1.State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
  2.University of Chinese Academy of Sciences, Beijing 100049, China
  3.Institute of Electronic Information Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China
- Author bio：
  
  SONG Lirong, lrsong@ihb.ac.cn
  GAN Nanqin, gannq@ihb.ac.cn
- Funds：
- DOI：10.1007/s00343-019-7186-1
  CLC：
- Received：19 June 2017，
  
  Accepted：28 November 2017，
  
  Online First：19 December 2017，
  
  Published：2018-07
- Accepted：
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Jun ZUO, Liting CHEN, Kun SHAN, et al. Assessment of different mcy genes for detecting the toxic to non-toxic Microcystis ratio in the field by multiplex qPCR[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(4): 1132-1144.
DOI：

Jun ZUO, Liting CHEN, Kun SHAN, et al. Assessment of different mcy genes for detecting the toxic to non-toxic Microcystis ratio in the field by multiplex qPCR[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(4): 1132-1144. DOI： 10.1007/s00343-019-7186-1.

摘要

Abstract

Harmful cyanobacterial blooms

especially

Microcystis

blooms

occur worldwide and draw widespread attention. The dynamics of microcystin-producing

Microcystis

and competition between microcystin-producing

Microcystis

and non-microcystin-producing

Microcystis

are key to predicting and treating

Microcystis

blooms. Multiplex qPCR is a useful tool to assess such issues. In this study

we developed multiplex qPCR methods with newly-designed probes and primers for the microcystin-synthesis related genes

mcyA

and

mcyE

. We used seven toxic

Microcystis

strains and four non-toxic

Microcystis

strains to compare the differences in the ratios of toxic and non-toxic

Microcystis

in mixed cultures

which were calculated using abundances of the genes

mcyA

mcyB

mcyD

mcyE

and phycocyanin (

). We also compared traditional cell counting and multiplex qPCR. Hierarchical clustering and principal component analysis indicated that

mcyD

was the most suitable

mcy

gene for quantification in laboratory experiments.

mcyB

abundances were always higher; we suggest that the amount of toxic

Microcystis

measured using

mcyB

might overestimate the actual percentages.

关键词

Keywords

references

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⁰

Assessment of different mcy genes for detecting the toxic to non-toxic Microcystis ratio in the field by multiplex qPCR

DOI：10.1007/s00343-019-7186-1

摘要

Abstract

关键词

Keywords

references