Preparation of low molecular weight <italic style="font-style: italic">Sargassum fusiforme</italic> polysaccharide and its anticoagulant activity

Yuhao SUN; Xiaolin CHEN; Song LIU; Huahua YU; Rongfeng LI; Xueqin WANG; Yukun QIN; Pengcheng LI

doi:10.1007/s00343-018-7089-6

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Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity

Biology | Updated：2023-05-19

- Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity
- Chinese Journal of Oceanology and Limnology Vol. 36, Issue 3, Pages: 882-891(2018)
- Affiliations：
  
  1.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  2.University of Chinese Academy of Sciences, Beijing 100049, China
- Author bio：
  
  CHEN Xiaolin, chenxl@qdio.ac.cn
  LI Pengcheng, pcli@qdio.ac.cn
- Funds：
- DOI：10.1007/s00343-018-7089-6
  CLC：
- Received：20 March 2017，
  
  Accepted：21 April 2017，
  
  Online First：27 May 2017，
  
  Published：2018-05
- Accepted：
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Yuhao SUN, Xiaolin CHEN, Song LIU, et al. Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(3): 882-891.
DOI：

Yuhao SUN, Xiaolin CHEN, Song LIU, et al. Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(3): 882-891. DOI： 10.1007/s00343-018-7089-6.

摘要

Abstract

Heparin has been used as an anticoagulant drug for many years

but it has significant side effects. In the search for good substitutes

low molecular weight (MW) polysaccharides from

Sargassum fusiforme

have been examined and confirmed to possess biological activities. Here

S. fusiforme

polysaccharides (SFP) were extracted and subjected to a hydrogen peroxide (H

) oxidation method for the preparation of low-MW SFP (LSFP). The effects of temperature

and H

concentration on the degradation process were also examined. Several LSFP of 36

5.7

and 2.7 kDa were obtained under different conditions

and their anticoagulant activities studied in vitro. The results showed that SFP and LSFP prolonged activated partial thromboplastin (APTT)

prothrombin (PT) and thrombin times (TT) significantly

indicating that these low MW polysaccharides possessed anticoagulant activity in the intrinsic

extrinsic

and common coagulation pathways. As these effects were related to the MW of the polysaccharides in APTT and TT but not in PT

the contents of the monosaccharide fucose and sulfate and the polysaccharide MW could have exerted combined effects. The details of this mechanism require further verification.

关键词

Keywords

references

Bouhlal R, Haslin C, Chermann J C, Colliec-Jouault S, Sinquin C, Simon G, Cerantola S, Riadi H, Bourgougnon N. 2011.Antiviral activities of sulfated polysaccharides isolated from Sphaerococcus coronopifolius ( Rhodophytha , Gigartinales ) and Boergeseniella thuyoides ( Rhodophyta , Ceramiales ). Marine Drugs, 9(12):1 187-1 209.

Camara R B G, Costa L S, Fidelis G P, Nobre L T D B, DantasSantos N, Cordeiro S L, Costa M S S P, Alves L G, Rocha H A O. 2011. Heterofucans from the brown seaweed Canistrocarpus cervicornis with anticoagulant and antioxidant activities. Marine Drugs, 9(1):124-138.

Chandía N P, Matsuhiro B. 2008. Characterization of a fucoidan from Lessonia vadosa (Phaeophyta) and its anticoagulant and elicitor properties. International Journal of Biological Macromolecules, 42(3):235-240.

Chen B J, Shi M J, Cui S, Hao S X, Hider R C, Zhou T. 2016.Improved antioxidant and anti-tyrosinase activity of polysaccharide from Sargassum fusiforme by degradation. International Journal of Biological Macromolecules, 92:715-722.

Chen H L, Zhang L, Long X E, Li P F, Chen S C, Kuang W, Guo J M. 2017. Sargassum fusiforme polysaccharides inhibit VEGF-A-related angiogenesis and proliferation of lung cancer in vitro and in vivo. Biomedicine & Pharmacotherapy, 85:22-27.

Chen S G, Xue C H, Yin L A, Tang Q J, Yu G L, Chai W G. 2011. Comparison of structures and anticoagulant activities of fucosylated chondroitin sulfates from different sea cucumbers. Carbohydrate Polymers, 83(2):688-696.

Chen X M, Nie W J, Yu G Q, Li Y L, Hu Y S, Lu J X, Jin L Q. 2012. Antitumor and immunomodulatory activity of polysaccharides from Sargassum fusiforme . Food and Chemical Toxicology, 50(3-4):695-700.

Cheng Z L, Wu X N. 2011. Study on the anticoagulant activities and promoting effect on human umbilical vein endothelial cells proliferation activity of fucoidan from Sargassum fusiforme Setchel . Food Research and Development, 32(4):165-167. (in Chinese with English abstract)

Dubois M, Gilles K A, Hamilton J K, Rebers P A, Smith F. 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28(3):350-356.

Guo H J, Liu Y Z, Paskaleva E E, Arra M, Kennedy J S, Shekhtman A, Canki M. 2014. Use of Sargassum fusiforme extract and its bioactive molecules to inhibit HIV infection:bridging two paradigms between eastern and western medicine. Chinese Herbal Medicines, 6(4):265-273.

Hou Y, Wang J, Jin W H, Zhang H, Zhang Q B. 2012.Degradation of Laminaria japonica fucoidan by hydrogen peroxide and antioxidant activities of the degradation products of different molecular weights. Carbohydrate Polymers, 87(1):153-159.

Hu P, Li Z X, Chen M C, Sun Z L, Ling Y, Jiang J, Huang C G. 2016. Structural elucidation and protective role of a polysaccharide from Sargassum fusiforme on ameliorating learning and memory deficiencies in mice. Carbohydrate Polymers, 139:150-158.

Jin W H, Zhang Q B, Wang J, Zhang W J. 2013. A comparative study of the anticoagulant activities of eleven fucoidans.Carbohydrate Polymers, 91(1):1-6.

Kawai Y, Seno N, Anno K. 1969. A modified method for chondrosulfatase assay. Analytical Biochemistry, 32(2):314-321.

Kim K I, Seo H D, Lee H S, Jo H Y, Yang H C. 1998. Studies on the blood anticoagulant polysaccharide isolated from hot water extracts of Hizikia fusiforme . Journal of the Korean Society of Food Science and Nutrition, 27(6):1 204-1 210.

Li B, Liu S, Xing R E, Li K C, Li R F, Qin Y K, Wang X Q, Wei Z H, Li P C. 2013. Degradation of sulfated polysaccharides from Enteromorpha prolifera and their antioxidant activities. Carbohydrate Polymers, 92(2):1 991-1 996.

Li N, Liu X, He X X, Wang S Y, Cao S J, Xia Z, Xian H L, Qin L, Mao W J. 2017. Structure and anticoagulant property of a sulfated polysaccharide isolated from the green seaweed Monostroma angicava . Carbohydrate Polymers, 159:195-206.

Liu C C, Zhou Y, Wu Y R, Gan J H, Zhou P G. 2004. Screening of the components with blood-anticoagulant activity from marine algae Sargassum fusiforme and Undaria pinnatifida . Journal of Fisheries of China, 28(4):473-476. (in Chinese with English abstract)

Melo F R, Pereira M S, Foguel D, Mourão P A S. 2004. Antithrombin-mediated anticoagulant activity of sulfated polysaccharides:different mechanisms for heparin and sulfated galactans. Journal of Biological Chemistry, 279(20):20 824-20 835.

Pang X X, Wang X. 2011. Research progress in tthrombosis and its mechanism. Medical Recapitulate, 17(11):1 613-1 616. (in Chinese with English abstract)

Qian W G, Li N, Lin L D, Xu T, Zhang X, Wang L H, Zou H X, Wu M J, Yan X F. 2016. Parallel analysis of proteins in brown seaweed Sargassum fusiforme responding to hyposalinity stress. Aquaculture, 465:189-197.

Song L, Chen X L, Liu X D, Zhang F B, Hu L F, Yue Y, Li K C, Li P C. 2016. Characterization and comparison of the structural features, immune-modulatory and anti-avian influenza virus activities conferred by three algal sulfated polysaccharides. Marine Drugs, 14(1):4.

Sudharsan S, Subhapradha N, Seedevi P, Shanmugam V, Madeswaran P, Shanmugam A, Srinivasan A. 2015. Antioxidant and anticoagulant activity of sulfated polysaccharide from Gracilaria debilis (Forsskal).International Journal of Biological Macromolecules, 81:1 031-1 038.

Sun L Q, Wang L, Zhou Y. 2012. Immunomodulation and antitumor activities of different-molecular-weight polysaccharides from Porphyridium cruentum .Carbohydrate Polymers, 87(2):1 206-1 210.

Tardy-Poncet B, Tardy B, Grelac F, Reynaud J, Mismetti P, Bertrand J C, Guyotat D. 1994. Pentosan polysulfateinduced thrombocytopenia and thrombosis. American Journal of Hematology, 45(3):252-257.

Wang J, Zhang Q B, Zhang Z S, Song H F, Li P C. 2010.Potential antioxidant and anticoagulant capacity of low molecular weight fucoidan fractions extracted from Laminaria japonica . International Journal of Biological Macromolecules, 46(1):6-12.

Wang J. 2010. Study on the Structure, Derivatives and Activities of Fucoidan Extracted from Laminia Japonica . University of Chinese Academy of Sciences (Institute of Oceanography), Beijing, China. p. 75-76. (in Chinese with English abstract)

Wang N, Feng Y F, Fan H P, Ma L, Wang D L, Wang D L, Ai Z L. 2013. Study on the difference of anticoagulant activity in vitro of crude polysaccharide of Jujube. Journal of Chinese Institute of Food Science and Technology, 13(12):34-39. (in Chinese with English abstract)

Wang S C, Bligh S W A, Shi S S, Wang Z T, Hu Z B, Crowder J, Branford-White C, Vella C. 2007. Structural features and anti-HIV-1 activity of novel polysaccharides from red algae Grateloupia longifolia and Grateloupia filicina .International Journal of Biological Macromolecules, 41(4):369-375.

Yue W, Yao P J, Wei Y A, Li S Q, Lai F, Liu X M. 2008. An innovative method for preparation of acid-free-watersoluble low-molecular-weight chitosan (AFWSLMWC).Food Chemistry, 108(3):1 082-1 087.

Zhang Z E, Liu X L, Yang J Y, Liu C C. 2008. Study on anticoagulant activity of sulfated polysaccharides from Hizikia fusiformis . Journal of Anhui Agricultural Sciences, 36(18):7 505-7 508, 7 542. (in Chinese with English abstract)

Zhang Z, Liu J G, Liu J D. 2002. Study review of Hizikia fusiformis . Marine Fisheries Research, 23(3):67-74. (in Chinese with English abstract)

Zhao X, Guo F J, Hu J, Zhang L J, Xue C H, Zhang Z H, Li B F. 2016. Antithrombotic activity of oral administered low molecular weight fucoidan from Laminaria japonica .Thrombosis Research, 144:46-52.

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Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity

DOI：10.1007/s00343-018-7089-6

摘要

Abstract

关键词

Keywords

references