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Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot
Schophthalmus maximus - Chinese Journal of Oceanology and Limnology Vol. 36, Issue 4, Pages: 1360-1373(2018)
- Affiliations：
1.Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
2.University of Chinese Academy of Sciences, Beijing 100049, China
3.Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China
4.Weihai Shenghang Aquatic Product Science and Technology Co. Ltd., Weihai 264200, China
- Author bio：
LIU Qinghua, qinghualiu@qdio.ac.cn
LI Jun, junli@qdio.ac.cn
- Funds：
DOI：10.1007/s00343-018-7068-y
CLC：Received：06 March 2017，
Accepted：07 May 2017，
Online First：06 June 2017，
Published：2018-07
- Accepted：
Scan QR Code
Chunyan ZHAO, Shihong XU, Chengcheng FENG, et al. Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot
Schophthalmus maximus [J]. Chinese Journal of Oceanology and Limnology, 2018, 36(4): 1360-1373.Chunyan ZHAO, Shihong XU, Chengcheng FENG, et al. Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot
Schophthalmus maximus [J]. Chinese Journal of Oceanology and Limnology, 2018, 36(4): 1360-1373. DOI： 10.1007/s00343-018-7068-y.
摘要
Abstract
Turbots (
Schophthalmus maximus
)
one of the most important economic marine flatfish species
fail to undergo final spawning and spermiation naturally under artificial farming conditions. In vertebrates
reproduction is regulated by the brain-pituitary-gonadal axis (BPG-axis)
and gonadotropin releasing hormone (GnRH) is one of its key components. Therefore
to better understand the physiology of reproduction in the turbot
three of the genes encoding GnRH subtypes-
sbGnRH
cGnRH-Ⅱ
and
sGnRH
-were cloned and sequenced by isolating the cDNA sequences. The localizations and patterns of expression of their mRNAs were also evaluated during seasonal gonadal development. All three mRNAs were expressed abundantly in the brain;
sbGnRH
and
sGnRH
mRNAs were also detected in the gonads and pituitary gland
and
sbGnRH
expression was much higher than that of
sGnRH
indicating the critical role of sbGnRH in regulating the BPG-axis. Moreover
the brain expression patterns of
sbGnRH
and
sGnRH
mRNAs showed an increased trend during gonadal development
peaking in mature stages. This indicated the direct regulation of gonadal development by the GnRH system. In addition
cGnRH-Ⅱ
mRNA expression showed no significant variations
suggesting that cGnRH-Ⅱ is not critically involved in the control of reproduction. Further
the mRNA abundances of the three GnRH forms in the breeding season were significantly higher than those in immature and post-breeding stages in all analyzed brain areas. Therefore
we propose that sbGnRH is the most important hormone for the regulation of reproduction in turbot via the BPG-axis. These results will help in better understanding the reproductive endocrine mechanisms of turbots and lay the groundwork for additional studies aimed at comparing the reproductive physiology of wild individuals with those raised under artificial conditions.
关键词
Keywords
references
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