Response of <italic style="font-style: italic">Microcystis aeruginosa</italic> FACHB-905 to different nutrient ratios and changes in phosphorus chemistry

Guotao PENG; W. WILHELM Steven; Sijie LIN; Xiangrong WANG

doi:10.1007/s00343-018-7065-1

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Response of Microcystis aeruginosa FACHB-905 to different nutrient ratios and changes in phosphorus chemistry

Updated：2023-05-19

- Response of Microcystis aeruginosa FACHB-905 to different nutrient ratios and changes in phosphorus chemistry
- Chinese Journal of Oceanology and Limnology Vol. 36, Issue 4, Pages: 1040-1052(2018)
- Affiliations：
  
  1.College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092, China
  2.Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China
  3.Department of Microbiology, University of Tennessee, Knoxville, TN 37920, USA
- Author bio：
  
  WANG Xiangrong, xrxrwang@fudan.edu.cn
- Funds：
- DOI：10.1007/s00343-018-7065-1
  CLC：
- Received：04 March 2017，
  
  Accepted：07 June 2017，
  
  Online First：06 September 2017，
  
  Published：2018-07
- Accepted：
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Guotao PENG, W. WILHELM Steven, Sijie LIN, et al. Response of Microcystis aeruginosa FACHB-905 to different nutrient ratios and changes in phosphorus chemistry[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(4): 1040-1052.
DOI：

Guotao PENG, W. WILHELM Steven, Sijie LIN, et al. Response of Microcystis aeruginosa FACHB-905 to different nutrient ratios and changes in phosphorus chemistry[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(4): 1040-1052. DOI： 10.1007/s00343-018-7065-1.

摘要

Abstract

Cyanobacterial blooms are a global problem

with their occurrence tightly tied to nutrient loading. We cultured

Microcystis aeruginosa

FACHB-905 in growth medium with either inorganic (orthophosphate) or organic (β-glycerophosphate or polyphosphate) phosphorus and at different N:P ratios with 50:1

30:1

16:1

4:1 and 1:4

serving as the phosphorus source. Fluorescence parameters were measured to determine the response of cellular responses to nutrient stress. Scanning electron microscopy (SEM) and estimates of antioxidant activity were employed to examine potential mechanisms of physical change. The results demonstrate that inorganic phosphorus was more bioavailable to

M. aeruginosa

relative to organic phosphorus in culture. The highest cell concentration (2.21×10

cells/mL)

chlorophyll-

(0.39 pg/cell) and phycocyanin (1.57 pg/cell) quotas and high levels of chlorophyll fluorescence parameters (rETR

PSⅡ

and

) were obtained when phosphorus was supplied as K

HPO

at a N:P ratio of 16-30. Organic sources of phosphorus (β-glycerophosphate and polyphosphate) were bioavailable to

M. aeruginosa

. In addition

too concentrated orthophosphate (N:P=1:4) resulted in the oxidative stress and lipid peroxidation of cell membrane (identified by the antioxidant system activity)

and the photosynthetic activity declined consequently. This study has demonstrated the effects of different phosphorus chemistries and N:P ratios on the cyanobacterial growth

photosynthetic activity and cell physiology

which could be an effective tool for predicting cyanobacterial dominance or N-deficiency in natural lakes (due to the superior ability of cyanobacteria for dissolved N and fix atmospheric N in some cases).

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references

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Response of Microcystis aeruginosa FACHB-905 to different nutrient ratios and changes in phosphorus chemistry

DOI：10.1007/s00343-018-7065-1

摘要

Abstract

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