Wen TENG, Rihao CONG, Huayong QUE, et al. De novo transcriptome sequencing reveals candidate genes involved in orange shell coloration of bay scallop Argopecten irradians[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(4): 1408-1416.
DOI:
Wen TENG, Rihao CONG, Huayong QUE, et al. De novo transcriptome sequencing reveals candidate genes involved in orange shell coloration of bay scallop Argopecten irradians[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(4): 1408-1416. DOI: 10.1007/s00343-018-7063-3.
De novo transcriptome sequencing reveals candidate genes involved in orange shell coloration of bay scallop Argopecten irradians
摘要
Abstract
Molluscan shell color has received persistent attention for its distinctive diversity and complexity. In the present study
six transcriptome libraries obtained from two developmental stages
pre-pigmentation and post-pigmentation
were used for paired-end sequencing in the bay scallop
Argopecten irradians
. In total
289 839 646 paired-end reads were assembled into 70 929 transcripts. Using BLASTX and BLASTN
30 896 unigenes were successfully annotated against the SWISS-PROT
NR
and KOG database. Gene ontology annotation and Kyoto Encyclopedia of Genes and Genomes classification identified numbers of unigenes involved in biomineralization and pigmentation. Digital gene expression analysis revealed that melanin
trace metal elements and porphyrins are potentially involved in shell coloration of
A. irradians
.
关键词
Keywords
references
Adamkewicz L, Castagna M. 1988. Genetics of shell color and pattern in the bay scallop Argopecten irradians . J. Hered., 79(1):14-17.
Altschul S F, Gish W, Miller W, Myers E W, Lipman D J. 1990.Basic local alignment search tool. J. Mol. Biol., 215(3):403-410.
Anders S, Huber W. 2010. Differential expression analysis for sequence count data. Genome Biol., 11(10):R106.
Arnow L E. 1938. The acid-soluble pigment of red human hair.Biochem. J., 32(8):1 281-1 284.
Bai Z Y, Zheng H F, Lin J Y, Wang G L, Li J L. 2013.Comparative analysis of the transcriptome in tissues secreting purple and white nacre in the pearl mussel Hyriopsis cumingii . PLoS One, 8(1):e53617.
Blake S G, Blake N J, Oesterling M J, Graves J E. 1997.Genetic divergence and loss of diversity in two cultured populations of the bay scallop, Argopecten irradians(Lamarck, 1819). J. Shellfish Res., 16:55-58.
Boutet E, Lieberherr D, Tognolli M, Schneider M, Bansal P, Bridge A J, Poux S, Bougueleret L, Xenarios I. 2016. UniProtKB/Swiss-Prot, the manually annotated section of the UniProt KnowledgeBase: how to use the entry view. In : Edwards D ed. Plant Bioinformatics. Methods in Molecular Biology. Humana Press, New York, USA. p. 23-54.
Cole T J. 1975. Inheritance of juvenile shell colour of the oyster drill Urosalpinx cinerea . Nature, 257(5529):794-795.
Comfort A. 1949. Acid-soluble pigments of shells. 1. The distribution of porphyrin fluorescence in molluscan shells.Biochem. J., 44(1):111-117.
Comfort A. 1951. The pigmentation of molluscan shells. Biol.Rev., 26(3):285-301.
Conesa A, Götz S, García-Gómez J M, Terol J, Talón M, Robles M. 2005. Blast2GO:a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics, 21(18):3 674-3 676.
Creese R G, Underwood A J. 1976. Observations on the biology of the trochid gastropod Austrocochlea constricta (Lamarck) (Prosobranchia). Ⅰ. Factors affecting shellbanding pattern. J. Exp. Mar. Biol. Ecol., 23(3):211-228.
Derby C D. 2014. Cephalopod ink:production, chemistry, functions and applications. Mar. Drugs, 12(5):2 700-2 730.
Ding J, Zhao L, Chang Y Q, Zhao W M, Du Z L, Hao Z L. 2015. Transcriptome sequencing and characterization of Japanese scallop Patinopecten yessoensis from different shell color lines. PLoS One, 10(2):e0116406.
Elek J A, Adamkewicz S L. 1990. Polymorphism for shell color in the Atlantic bay scallop Argopecten irradians irradians (Lamarck) (Mollusca:Bivalvia) on Martha's Vineyard Island. Am. Malacol. Bull., 7:117-126.
Fox D L. 1976. Animal biochromes and structural colours: physical, chemical, distributional & physiological features of coloured bodies in the animal world. 2 nd edn. University of California Press, California, USA.
Grabherr M G, Haas B J, Yassour M, Levin J Z, Thompson D A, Amit I, Adiconis X, Fan L, Raychowdhury R, Zeng Q D, Chen Z H, Mauceli E, Hacohen N, Gnirke A, Rhind N, di Palma F, Birren B W, Nusbaum C, Lindblad-Toh K, Friedman N, Regev A. 2011. Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat. Biotechnol., 29(7):644-652.
Guan Y Y, Huang L M, He M X. 2011. Construction of cDNA subtractive library from pearl oyster ( Pinctada fucata Gould ) with red color shell by SSH. Chin. J. Oceanol.Limnol., 29(3):616-622.
Haas B J, Papanicolaou A, Yassour M, Grabherr M, Blood P D, Bowden J, Couger M B, Eccles D, Li B, Lieber M, MacManes M D, Ott M, Orvis J, Pochet N, Strozzi F, Weeks N, Westerman R, William T, Dewey C N, Henschel R, LeDuc R D, Friedman N, Regev A. 2013. De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis.Nat. Protoc., 8(8):1 494-1 512.
Hao S X, Hou X, Wei L, Li J, Li Z H, Wang X T. 2015.Extraction and identification of the pigment in the adductor muscle scar of Pacific oyster Crassostrea gigas .PLoS One, 10(11):e0142439.
Ino T. 1949. The effect of food on growth and coloration of the topshell ( Turbo cornutus Solander ). J. Mar. Res., 8:1-5.
Kobayashi T, Urabe K, Winder A, Jiménez-Cervantes C, Imokawa G; Brewington T, Solano F, García-Borrón J C, Hearing V J. 1994. Tyrosinase related protein 1 (TRP1)functions as a DHICA oxidase in melanin biosynthesis.EMBO J., 13(24):5 818-5 825.
Langmead B, Salzberg S L. 2012. Fast gapped-read alignment with Bowtie 2. Nat. Methods, 9(4):357-359.
Leighton D L. 1961. Observations of the effect of diet on shell coloration in the red abalone, Haliotis rufescens Swainson.Veliger, 4:29-32.
Moriya Y, Itoh M, Okuda S, Yoshizawa A, Kanehisa M. 2007.KAAS:an automatic genome annotation and pathway reconstruction server. Nucleic Acids Res., 35(S2):W182-W185.
Mortazavi A, Williams B A, McCue K, Schaeffer L, Wold B. 2008. Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat. Methods, 5(7):621-628.
Newkirk G F. 1980. Genetics of shell color in Mytilus edulis L.and the association of growth rate with shell color. J. Exp.Mar. Biol. Ecol., 47(1):89-94.
Palmer A R. 1985. Genetic basis of shell variation in Thais emarginata (Prosobranchia, Muricacea). Ⅰ. Banding in populations from Vancouver Island. Biol. Bull., 169(3):638-651.
Patel R K, Jain M. 2012. NGS QC Toolkit:a toolkit for quality control of next generation sequencing data. PLoS One, 7(2):e30619.
Pertea G, Huang X Q, Liang F, Antonescu V, Sultana R, Karamycheva S, Lee Y, White J, Cheung F, Parvizi B, Tsai J, Quackenbush J. 2003. TIGR Gene Indices clustering tools (TGICL):a software system for fast clustering of large EST datasets. Bioinformatics, 19(5):651-652.
Qin Y J, Liu X, Zhang H B, Zhang G F, Guo X M. 2007.Identification and mapping of amplified fragment length polymorphism markers linked to shell color in bay scallop, Argopecten irradians irradians (Lamarck, 1819).Mar. Biotechnol., 9(1):66-73.
Roberts A, Pachter L. 2012. Streaming fragment assignment for real-time analysis of sequencing experiments. Nat.Methods, 10(1):71-73.
Sánchez-Ferrer A, Rodríguez-López J N, García-Cánovas F, García-Carmona F. 1995. Tyrosinase:a comprehensive review of its mechanism. Biochim. Biophys. Acta, 1247(1):1-11.
Tatusov R L, Fedorova N D, Jackson J D, Jacobs A R, Kiryutin B, Koonin E V, Krylov D M, Mazumder R, Mekhedov S L, Nikolskaya A N, Rao B S, Smirnov S, Sverdlov A V, Vasudevan S, Wolf Y I, Yin J J, Natale D A. 2003. The COG database:an updated version includes eukaryotes.BMC Bioinformatics, 4:41.
Verdes A, Cho W, Hossain M, Brennan P L R, Hanley D, Grim T, Hauber M E, Holford M. 2015. Nature's palette:characterization of shared pigments in colorful avian and mollusk shells. PLos One, 10(12):e0143545.
Williams S T, Ito S, Wakamatsu K, Goral T, Edwards N P, Wogelius R A, Henkel T, de Oliveira L F, Maia L F, Strekopytov S. 2016. Identification of shell colour pigments in marine snails Clanculus pharaonius and C.margaritarius (Trochoidea; Gastropoda). PLos One, 11(7):e0156664.
Winkler F M, Estévez B F, Jollán L B, Garrido J P. 2001.Inheritance of the general shell color in the scallop Argopecten purpuratus (Bivalvia:Pectinidae). J. Hered., 92(6):521-525.
Yue X, Nie Q, Xiao G Q, Liu B Z. 2015. Transcriptome analysis of shell color-related genes in the clam Meretrix meretrix . Mar. Biotechnol., 17(3):364-374.
Zhang F S, He Y C, Liu X S, Ma J H, Li S Y, Qi L X. 1986. A report on the introduction, spat-rearing and experimental culture of bay scallop, Argopecten irradians Lamarck.Oceanologia et Limnologia Sinica, 17(5):367-374. (in Chinese with English abstract)
Zheng H P, Zhang T, Sun Z W, Liu W H, Liu H L. 2013.Inheritance of shell colours in the noble scallop Chlamys nobilis (Bivalve:Pectinidae). Aquac. Res., 44(8):1 229-1 235.
Zviak C, Milléquant J. 2005. Oxidation coloring. In : Bouillon C, Wilkinson J eds. The Science of Hair Care. 2 nd edn. CRC Press, Boca Raton, USA. p. 251-276.
The trial reading is over, you can activate your VIP account to continue reading.
Transcriptome analysis reveals immune-related genes in tissues of Vibrio anguillarum-infected turbot Scophthalmus maximus
Comparative transcriptomic analysis of Macrobrachium nipponense in response to Aeromonas veronii or Staphylococcus aureus infection
Comparative transcriptome analysis of abalone Haliotis discus hannai with green and gray egg colors
Eyes of differing colors in Alvinocaris longirostris from deep-sea chemosynthetic ecosystems: genetic and molecular evidence of its formation mechanism
Transcriptional responses to starvation of pathogenic Vibrio harveyi strain DY1
Related Author
Guobin HU
Xianzhi DONG
Maqsood Ahmed SOOMRO
Yuting SONG
Zhipeng ZHANG
Chong HAN
Qiang LI
Qinghua CHEN
Related Institution
Institute of Biophysis, Chinese Academy of Sciences
Institute of Evolution & Marine Biodiversity, Ocean University of China
College of Marine Life Sciences, Ocean University of China
Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai)
State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of life Sciences, Sun Yat-Sen University