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Denitrification potential evaluation of a newly indigenous aerobic denitrifier isolated from largemouth bass
Micropterus salmoides culture pond- Chinese Journal of Oceanology and Limnology Vol. 36, Issue 3, Pages: 913-925(2018)
- Affiliations：
1.Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China
2.College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China
- Author bio：
XIE Jun, xiejunhy01@126.com
- Funds：
DOI：10.1007/s00343-018-7029-5
CLC：Received：30 March 2017，
Accepted：04 May 2017，
Online First：27 May 2017，
Published：2018-05
- Accepted：
Scan QR Code
Cuicui WANG, Kai ZHANG, Jun XIE, et al. Denitrification potential evaluation of a newly indigenous aerobic denitrifier isolated from largemouth bass
Micropterus salmoides culture pond[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(3): 913-925.Cuicui WANG, Kai ZHANG, Jun XIE, et al. Denitrification potential evaluation of a newly indigenous aerobic denitrifier isolated from largemouth bass
Micropterus salmoides culture pond[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(3): 913-925. DOI： 10.1007/s00343-018-7029-5.
摘要
Abstract
This work evaluates the application potential of a new indigenous aerobic denitrifier
strain
Pseudomonas
CW-2
isolated from a largemouth bass culture pond. The rate of ammonium-N removal by strain CW-2 was approximately 97% at a DO concentration of 5.2 mg/L. Furthermore
when nitrate and ammonia coexisted
the strain gave priority to assimilating ammonia
and thereafter to denitrification. Under optimal cultivation conditions
citrate and acetate were the carbon resources
C/N was 8
dissolved oxygen was 5.2 mg/L
and pH was 7; the removal rate of ammonium reached nearly 90%. The changing patterns of different bacteria in strain CW-2-treated and the control pond water were also compared. Lower levels of ammonia
nitrite
and phosphates were observed in the treated water as compared with the controls. Meanwhile
phylum-level distributions of the bacterial OTUs revealed that
Proteobacteria
Bacteroidetes
Planctomycetes
and
Nitrospirae
continuously changed their relative abundances in relation to carbon and the addition of strain CW-2; this finding implies that the conventional denitrification process was weakened under the effects of carbon or the presence of strain CW-2. We propose that strain CW-2 is a promising organism for the removal of ammonium in intensive fish culture systems
according to our evaluations of its denitrification performance.
关键词
Keywords
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