A novel male-associated marker for sex identification in Ussuri catfish <italic style="font-style: italic">Pseudobagrus ussuriensis</italic>

Chuankun ZHU; Zhengjun PAN; Hui WANG; Guoliang CHANG; Huaiyu DING

doi:10.1007/s00343-018-6342-3

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A novel male-associated marker for sex identification in Ussuri catfish Pseudobagrus ussuriensis

Biology | Updated：2023-05-19

- A novel male-associated marker for sex identification in Ussuri catfish Pseudobagrus ussuriensis
- Chinese Journal of Oceanology and Limnology Vol. 36, Issue 3, Pages: 850-852(2018)
- Affiliations：
  
  1.Jiangsu Engineering Laboratory for Breeding of Special Aquatic Organisms, Huaiyin Normal University, Huai'an 223300, China
  2.Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection, Huaiyin Normal University, Huai'an 223300, China
- Author bio：
  
  ZHU Chuankun, zhuchuankun@hytc.edu.cn
- Funds：
- DOI：10.1007/s00343-018-6342-3
  CLC：
- Received：13 January 2017，
  
  Accepted：27 February 2017，
  
  Online First：25 April 2017，
  
  Published：2018-05
- Accepted：
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Chuankun ZHU, Zhengjun PAN, Hui WANG, et al. A novel male-associated marker for sex identification in Ussuri catfish Pseudobagrus ussuriensis[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(3): 850-852.
DOI：

Chuankun ZHU, Zhengjun PAN, Hui WANG, et al. A novel male-associated marker for sex identification in Ussuri catfish Pseudobagrus ussuriensis[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(3): 850-852. DOI： 10.1007/s00343-018-6342-3.

摘要

Abstract

The Ussuri catfish

Pseudobagrus ussuriensis

is a promising aquaculture fish with sexual dimorphism that males grow 2-3 times faster than females. Therefore

all-male breeding could increase production of this fish

and sex-linked markers would be useful during the breeding process. In this study

a male-associated marker named

PuGT54

was isolated in

P. ussuriensis

from a previously constructed microsatellite library. A total of 185

P. ussuriensis

individuals containing 95 females and 90 males were used to test the usage of this maker. And results showed that sexes of all individuals were successfully identified

indicating the high reliability and accuracy of this marker for sex identification. Furthermore

the male-specific fragment amplified by this maker was cloned and sequenced

which was 118 base pairs in length. As homologous genes of this fragment was not found through BLASTn

detailed information of the male-specific fragment is unknown presently. Nevertheless

this marker would be helpful in conservation

breeding and ecological study of

P. ussuriensis

关键词

Keywords

references

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A novel male-associated marker for sex identification in Ussuri catfish Pseudobagrus ussuriensis

DOI：10.1007/s00343-018-6342-3

摘要

Abstract

关键词

Keywords

references