Development of novel microsatellite markers for <italic style="font-style: italic">Holothurian scabra</italic> (Holothuriidae), <italic style="font-style: italic">Apostichopus japonicas</italic> (Stichopodidae) and cross-species testing in other sea cucumbers

Jingbo SHANGGUAN; Zhongbao LI

doi:10.1007/s00343-018-6315-6

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Development of novel microsatellite markers for Holothurian scabra (Holothuriidae), Apostichopus japonicas (Stichopodidae) and cross-species testing in other sea cucumbers

Biology | Updated：2023-05-19

- Development of novel microsatellite markers for Holothurian scabra (Holothuriidae), Apostichopus japonicas (Stichopodidae) and cross-species testing in other sea cucumbers
- Chinese Journal of Oceanology and Limnology Vol. 36, Issue 2, Pages: 519-527(2018)
- Affiliations：
  
  1.Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-Environment, Xiamen 361021, China
  2.Fisheries College, Jimei University, Xiamen 361021, China
- Author bio：
  
  LI Zhongbao, lizhongbao@jmu.edu.cn
- Funds：
- DOI：10.1007/s00343-018-6315-6
  CLC：
- Received：03 December 2016，
  
  Accepted：31 December 2016，
  
  Online First：03 February 2017，
  
  Published：2018-03
- Accepted：
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Jingbo SHANGGUAN, Zhongbao LI. Development of novel microsatellite markers for Holothurian scabra (Holothuriidae), Apostichopus japonicas (Stichopodidae) and cross-species testing in other sea cucumbers[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(2): 519-527.
DOI：

Jingbo SHANGGUAN, Zhongbao LI. Development of novel microsatellite markers for Holothurian scabra (Holothuriidae), Apostichopus japonicas (Stichopodidae) and cross-species testing in other sea cucumbers[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(2): 519-527. DOI： 10.1007/s00343-018-6315-6.

摘要

Abstract

Thirty-five new microsatellite loci from the sea cucumbers

Holothurian scabra

(Jaeger

1833) and

Apostichopus japonicas

(Selenka

1867) were screened and characterized using the method of magnetic bead enrichment. Of the twenty-four polymorphic loci tested

eighteen were consistent with Hardy-Weinberg equilibrium after a modified false discovery rate (B-Y FDR) correction

whereas six showed statistically significant deviations (CHS2 and CHS11:

0.014 790; FCS1

FCS6

FCS8 and FCS14:

0.015 377). Furthermore

four species of plesiomorphous and related sea cucumbers (

Holothurian scabra

Holothuria leucospilota

Stichopus horrens

and

Apostichopus japonicas

) were tested for mutual cross-amplification using a total of ninety microsatellite loci. Although transferability and universality of all loci were generally low

the results of the cross-species study showed that the markers can be applied to identify individuals to species according to the presence or absence of specific microsatellite alleles. The microsatellite markers reported here will contribute to the study of genetic diversity

assisted breeding

and population conservation in sea cucumbers

as well as allow for the identification of individuals to closely related species.

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Keywords

references

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Development of novel microsatellite markers for Holothurian scabra (Holothuriidae), Apostichopus japonicas (Stichopodidae) and cross-species testing in other sea cucumbers

DOI：10.1007/s00343-018-6315-6

摘要

Abstract

关键词

Keywords

references