Nutrient-enhanced <italic style="font-style: italic">n</italic>-alkanes biodegradation and succession of bacterial communities

Yanyu SUN; Hui WANG; Junde LI; Bin WANG; Cancan QI; Xiaoke HU

doi:10.1007/s00343-018-6310-y

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Nutrient-enhanced n-alkanes biodegradation and succession of bacterial communities

Updated：2023-05-19

- Nutrient-enhanced n-alkanes biodegradation and succession of bacterial communities
- Chinese Journal of Oceanology and Limnology Vol. 36, Issue 4, Pages: 1294-1303(2018)
- Affiliations：
  
  1.Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Costal Zone Research, Chinese Academy of Sciences, Yantai 264003, China
  2.University of Chinese Academy of Sciences, Beijing 100049, China
- Author bio：
  
  HU Xiaoke, xkhu@yic.ac.cn
- Funds：
- DOI：10.1007/s00343-018-6310-y
  CLC：
- Received：09 December 2016，
  
  Accepted：17 April 2017，
  
  Online First：07 June 2017，
  
  Published：2018-07
- Accepted：
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Yanyu SUN, Hui WANG, Junde LI, et al. Nutrient-enhanced n-alkanes biodegradation and succession of bacterial communities[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(4): 1294-1303.
DOI：

Yanyu SUN, Hui WANG, Junde LI, et al. Nutrient-enhanced n-alkanes biodegradation and succession of bacterial communities[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(4): 1294-1303. DOI： 10.1007/s00343-018-6310-y.

摘要

Abstract

Bioremediation

is an effective and environment-friendly method of cleaning up crude oil pollution after an oil spill. However

the in situ bioremediation of crude oil is usually inhibited by deficiency of inorganic nutrients. To understand the effects of nutrient addition on the bioremediation of crude oil and the succession of bacterial communities during process of bioremediation

microcosms containing oilcontaminated sediments were constructed and biodegradation of crude oil was assessed based on the depletion of different ingredients. We used two culture-independent methods

denaturing gradient gel electrophoresis and a 16S rRNA gene based clone library

to analyze the succession of bacterial communities. The results suggested

-alkanes were degraded after 30 days and that nutrient amendments significantly improved the efficiency of their biodegradation. Moreover

oil contamination and nutrient amendments could dramatically change bacterial community structures. Lower diversity was detected after being contaminated with oil. For instance

bacterial clones affiliated with the phylum Armatimonadetes

Firmicutes

Gemmatimonadetes

and Planctomycetes and the class Deltaproteobacteria and Epsilonproteobacteria could not be identified after 30 days of incubation with crude oil. However

"professional hydrocarbonocastic bacteria" became abundant in samples treated with oil during the bioremediation period

while these clones were almost completely absent from the control plots. Interestingly

bioinformatics analysis showed that even when dramatic differences in oil biodegradation efficiency were observed

bacterial communities in the plots with nutrient amendments were not significantly different from those in plots treated with oil alone. These findings indicated that nutrient amendments could stimulate the process of biodegradation but had less impact on bacterial communities. Overall

nutrient amendments might be able to stimulate the growth of n-alkane degrading bacteria.

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Keywords

references

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Related Institution

School of Ocean, Yantai University

Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences

Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences

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Nutrient-enhanced n-alkanes biodegradation and succession of bacterial communities

DOI：10.1007/s00343-018-6310-y

摘要

Abstract

关键词

Keywords

references