Characterization of a novel deep-sea microbial esterase EstC10 and its use in the generation of (<italic style="font-style: italic">R</italic>)-methyl 2-chloropropionate

Yanhui GONG; Sanmei MA; Yongfei WANG; Yongkai XU; Aijun SUN; Yun ZHANG; Yunfeng HU

doi:10.1007/s00343-018-6297-4

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Characterization of a novel deep-sea microbial esterase EstC10 and its use in the generation of (R)-methyl 2-chloropropionate

Biology | Updated：2023-05-19

- Characterization of a novel deep-sea microbial esterase EstC10 and its use in the generation of (R)-methyl 2-chloropropionate
- Chinese Journal of Oceanology and Limnology Vol. 36, Issue 2, Pages: 473-482(2018)
- Affiliations：
  
  1.Key Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
  2.Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
  3.Department of Biotechnology, Jinan University, Guangzhou 510632, China
  4.Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250014, China
  5.South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou 510275, China
- Author bio：
  
  HU Yunfeng, yunfeng.hu@scsio.ac.cn
- Funds：
- DOI：10.1007/s00343-018-6297-4
  CLC：
- Received：16 November 2016，
  
  Accepted：17 January 2017，
  
  Online First：14 February 2018，
  
  Published：2018-03
- Accepted：
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Yanhui GONG, Sanmei MA, Yongfei WANG, et al. Characterization of a novel deep-sea microbial esterase EstC10 and its use in the generation of (R)-methyl 2-chloropropionate[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(2): 473-482.
DOI：

Yanhui GONG, Sanmei MA, Yongfei WANG, et al. Characterization of a novel deep-sea microbial esterase EstC10 and its use in the generation of (R)-methyl 2-chloropropionate[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(2): 473-482. DOI： 10.1007/s00343-018-6297-4.

摘要

Abstract

A novel esterase EstC10 from

Bacillus

sp. CX01 isolated from the deep sea of the Western Pacific Ocean and the functionalities of EstC10 was characterized. At present

the reports about the kinetic resolution of racemic methyl 2-chloropropionate were quite rare. So we developed deep-sea microbial esterase EstC10 as a novel biocatalyst in the kinetic resolution of racemic methyl 2-chloropropionate and generate (

)-methyl 2-chloropropionate with high enantiomeric excess (

99%) after the optimization of process parameters such as pH

temperature

organic co-solvents

surfactants

substrate concentration and reaction time. Notably

the optimal substrate concentration (80 mmol/L) of esterase EstC10 was higher than the kinetic resolution of another esterase

Est12-7 (50 mmol/L). The novel microbial esterase EstC10 identified from the deep sea was a promising green biocatalyst in the generation of (

)-methyl 2-chloropropionate as well of many other valuable chiral chemicals in industry.

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references

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Characterization of a novel deep-sea microbial esterase EstC10 and its use in the generation of (R)-methyl 2-chloropropionate

DOI：10.1007/s00343-018-6297-4

摘要

Abstract

关键词

Keywords

references