Fish-mediated changes in bacterioplankton community composition: an in situ mesocosm experiment

Congqiang LUO; Chunlong YI; Leyi NI; Longgen GUO

doi:10.1007/s00343-018-6281-z

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Fish-mediated changes in bacterioplankton community composition: an in situ mesocosm experiment

Ecology | Updated：2023-05-19

- Fish-mediated changes in bacterioplankton community composition: an in situ mesocosm experiment
- Chinese Journal of Oceanology and Limnology Vol. 36, Issue 2, Pages: 341-350(2018)
- Affiliations：
  
  1.Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
  2.Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Key Laboratory of Health Aquaculture and Product Processing in Dongting Lake Area of Hunan Province, Hunan University of Arts and Science, Changde 415000, China
- Author bio：
  
  GUO Longgen, longgen@ihb.ac.cn
- Funds：
- DOI：10.1007/s00343-018-6281-z
  CLC：
- Received：11 October 2016，
  
  Accepted：04 January 2017，
  
  Online First：10 February 2017，
  
  Published：2018-02
- Accepted：
Scan QR Code
Congqiang LUO, Chunlong YI, Leyi NI, et al. Fish-mediated changes in bacterioplankton community composition: an in situ mesocosm experiment[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(2): 341-350.
DOI：

Congqiang LUO, Chunlong YI, Leyi NI, et al. Fish-mediated changes in bacterioplankton community composition: an in situ mesocosm experiment[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(2): 341-350. DOI： 10.1007/s00343-018-6281-z.

摘要

Abstract

We characterized variations in bacterioplankton community composition (BCC) in mesocosms subject to three different treatments. Two groups contained fish (group one:

Cyprinus carpio

; group two:

Hypophthalmichthys molitrix

); and group three

the untreated mesocosm

was the control. Samples were taken seven times over a 49-d period

and BCC was analyzed by PCR-denaturing gradient gel electrophoresis (DGGE) and real-time quantitative PCR (qPCR). Results revealed that introduction of

C. carpio

and

H. molitrix

had a remarkable impact on the composition of bacterioplankton communities

and the BCC was significantly different between each treatment. Sequencing of DGGE bands revealed that the bacterioplankton community in the different treatment groups was consistent at a taxonomic level

but differed in its abundance.

H. molitrix

promoted the richness of Alphaproteobacteria and Actinobacteria

while more bands affiliated to Cyanobacteria were detected in

C. carpio

mesocosms. The redundancy analysis (RDA) result demonstrated that the BCC was closely related to the bottom-up (total phosphorus

chlorophyll a

phytoplankton biomass) and top-down forces (biomass of copepods and cladocera) in

C. carpio

and control mesocosms

respectively. We found no evidence for top-down regulation of BCC by zooplankton in

H. molitrix

mesocosms

while grazing by protozoa (heterotrophic nanoflagellates

ciliates) became the major way to regulate BCC. Total bacterioplankton abundances were significantly higher in

C. carpio

mesocosms because of high nutrient concentration and suspended solids. Our study provided insights into the relationship between fish and bacterioplankton at species level

leading to a deep understanding of the function of the microbial loop and the aquatic ecosystem.

关键词

Keywords

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Related Author

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Related Institution

Donghu Experimental Station of Lake Ecosystems State Key Laboratory of Freshwater Ecology and Biotechnology of China Institute of Hydrobiology Chinese Academy of Sciences

University of Chinese Academy of Sciences

College of Marine Life Sciences, Ocean University of China

Key Laboratory of Marine Genetics and Breeding(OUC), Ministry of Education

Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology

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