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Effects of elevated
p CO2 on physiological performance of marine microalgaeDunaliella salina (Chlorophyta, Chlorophyceae)- Chinese Journal of Oceanology and Limnology Vol. 36, Issue 2, Pages: 317-328(2018)
- Affiliations：
1.College of Marine Life Science, Ocean University of China, Qingdao 266003, China
2.Rongcheng Ocean and Fisheries Bureau, Weihai 264300, China
- Author bio：
TANG Xuexi, tangxx@ouc.edu.cn
- Funds：
DOI：10.1007/s00343-018-6278-7
CLC：Received：08 October 2016，
Accepted：02 December 2016，
Online First：04 February 2017，
Published：2018-02
- Accepted：
Scan QR Code
Shunxin HU, You WANG, Ying WANG, et al. Effects of elevated
p CO2 on physiological performance of marine microalgaeDunaliella salina (Chlorophyta, Chlorophyceae)[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(2): 317-328.Shunxin HU, You WANG, Ying WANG, et al. Effects of elevated
p CO2 on physiological performance of marine microalgaeDunaliella salina (Chlorophyta, Chlorophyceae)[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(2): 317-328. DOI： 10.1007/s00343-018-6278-7.
摘要
Abstract
The present study was conducted to determine the effects of elevated
p
CO
2
on growth
photosynthesis
dark respiration and inorganic carbon acquisition in the marine microalga
Dunaliella salina
. To accomplish this
D. salina
was incubated in semi-continuous cultures under present-day CO
2
levels (390 μatm
pH
NBS
:8.10)
predicted year 2100 CO
2
levels (1 000 μatm
pH
NBS
:7.78) and predicted year 2300 CO
2
levels (2 000 μatm
pH
NBS
:7.49). Elevated
p
CO
2
significantly enhanced photosynthesis (in terms of gross photosynthetic O
2
evolution
effective quantum yield (△
F
/
F
'
m
)
photosynthetic efficiency (
α
)
maximum relative electron transport rate (rETR
max
) and ribulose-1
5-bisphosphate carboxylase/oxygenase (Rubisco) activity) and dark respiration of
D. salina
but had insignificant effects on growth. The photosynthetic O
2
evolution of
D. salina
was significantly inhibited by the inhibitors acetazolamide (AZ)
ethoxyzolamide (EZ) and 4
4'-diisothiocyanostilbene-2
2'-disulfonate (DIDS)
indicating that
D. salina
is capable of acquiring HCO
3
-
via extracellular carbonic anhydrase and anion-exchange pr 3 oteins. Furthermore
the lower inhibition of the photosynthetic O
2
evolution at high
p
CO
2
levels by AZ
EZ and DIDS and the decreased carbonic anhydrase showed that carbon concentrating mechanisms were down-regulated at high
p
CO
2
. In conclusion
our results show that photosynthesis
dark respiration and CCMs will be affected by the increased
p
CO
2
/low pH conditions predicted for the future
but that the responses of
D. salina
to high
p
CO
2
/low pH might be modulated by other environmental factors such as light
nutrients and temperature. Therefore
further studies are needed to determine the interactive effects of
p
CO
2
temperature
light and nutrients on marine microalgae.
关键词
Keywords
references
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