Molecular cloning and transcriptional analysis of a NPY receptor-like in common Chinese cuttlefish <italic style="font-style: italic">Sepiella japonica</italic>

Jingwen YANG; Yuchao XU; Ke XU; Hongling PING; Huilai SHI; Zhenming LÜ; Changwen WU; Tianming WANG

doi:10.1007/s00343-018-6270-2

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Molecular cloning and transcriptional analysis of a NPY receptor-like in common Chinese cuttlefish Sepiella japonica

Biology | Updated：2023-05-19

- Molecular cloning and transcriptional analysis of a NPY receptor-like in common Chinese cuttlefish Sepiella japonica
- Chinese Journal of Oceanology and Limnology Vol. 36, Issue 3, Pages: 892-904(2018)
- Affiliations：
  
  1.National Engineering Research Center for Facilitated Marine Aquaculture, Marine Science College, Zhejiang Ocean University, Zhoushan 316022, China
  2.Marine Fisheries Research Institute of Zhejiang Province, Zhoushan 316022, China
- Author bio：
  
  WANG Tianming, wtmzjuedu@163.com, wangtianming@zjou.edu.cn
- Funds：
- DOI：10.1007/s00343-018-6270-2
  CLC：
- Received：14 October 2016，
  
  Accepted：08 December 2016，
  
  Online First：20 March 2017，
  
  Published：2018-05
- Accepted：
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Jingwen YANG, Yuchao XU, Ke XU, et al. Molecular cloning and transcriptional analysis of a NPY receptor-like in common Chinese cuttlefish Sepiella japonica[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(3): 892-904.
DOI：

Jingwen YANG, Yuchao XU, Ke XU, et al. Molecular cloning and transcriptional analysis of a NPY receptor-like in common Chinese cuttlefish Sepiella japonica[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(3): 892-904. DOI： 10.1007/s00343-018-6270-2.

摘要

Abstract

Neuropeptide Y (NPY) has a pivotal role in the regulation of many physiological processes. In this study

the gene encoding a NPY receptor-like from the common Chinese cuttlefish

Sepiella japonica

(

NPYR-like) was identified and characterized. The full-length

SjNPYR-like

cDNA was cloned containing a 492-bp of 5' untranslated region (UTR)

1 182 bp open reading frame (ORF) encoding a protein of 393 amino acid residues

and 228 bp of 3' UTR. The putative protein was predicted to have a molecular weight of 45.54 kDa and an isoelectric point (pI) of 8.13. By informatic analyses

NPYR-like was identified as belonging to the class A G protein coupled receptor (GPCR) family (the rhodopsin-type). The amino acid sequence contained 12 potential phosphorylation sites and five predicted N-linked glycosylation sites. Multiple sequence alignment and 3D structure modeling were conducted to clarify

NPYR bioinformatics characteristics. Phylogenetic analysis identifies it as an NPYR with identity of 33% to

Lymnaea stagnalis

NPFR. Transmembrane properties of

NPYR-like were demonstrated in vitro using HEK293 cells and the pEGFP-N1 plasmid. Relative quantification of

NPYR-like mRNA level confirmed a high level expression and broad distribution of

SjNPYR-like

in various tissues of female

S. japonica

. In addition

the transcriptional profile of

SjNPYR-like

in the brain

liver

and ovary during gonadal development was analyzed. The results provide basic understanding on the molecular characteristics of

NPYR-like and its potentially physical functions.

关键词

Keywords

references

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Molecular cloning and transcriptional analysis of a NPY receptor-like in common Chinese cuttlefish Sepiella japonica

DOI：10.1007/s00343-018-6270-2

摘要

Abstract

关键词

Keywords

references