Molecular characterization and expression analysis of Lilytype lectin (<italic style="font-style: italic">Sm</italic>LTL) in turbot <italic style="font-style: italic">Scophthalmus maximus</italic>, and its response to <italic style="font-style: italic">Vibrio anguillarum</italic>

Dandan XIA; Aijun MA; Zhihui HUANG; Xiaomei SHANG; Wenxiao CUI; Zhi YANG; Jiangbo QU

doi:10.1007/s00343-017-6268-1

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Molecular characterization and expression analysis of Lilytype lectin (SmLTL) in turbot Scophthalmus maximus, and its response to Vibrio anguillarum

Biology | Updated：2023-05-19

- Molecular characterization and expression analysis of Lilytype lectin (SmLTL) in turbot Scophthalmus maximus, and its response to Vibrio anguillarum
- Chinese Journal of Oceanology and Limnology Vol. 36, Issue 2, Pages: 508-518(2018)
- Affiliations：
  
  1.Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture; Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology; Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
  2.Ocean University of Shanghai, Shanghai 201306, China
  3.Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China
  4.Yantai Tianyuan Aquatic Limited Corporation, Yantai 264006, China
- Author bio：
  
  MA Aijun, maaj@ysfri.ac.cn
- Funds：
- DOI：10.1007/s00343-017-6268-1
  CLC：
- Received：03 October 2016，
  
  Accepted：31 October 2016，
  
  Online First：30 November 2016，
  
  Published：2018-03
- Accepted：
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Dandan XIA, Aijun MA, Zhihui HUANG, et al. Molecular characterization and expression analysis of Lilytype lectin (SmLTL) in turbot Scophthalmus maximus, and its response to Vibrio anguillarum[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(2): 508-518.
DOI：

Dandan XIA, Aijun MA, Zhihui HUANG, et al. Molecular characterization and expression analysis of Lilytype lectin (SmLTL) in turbot Scophthalmus maximus, and its response to Vibrio anguillarum[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(2): 508-518. DOI： 10.1007/s00343-017-6268-1.

摘要

Abstract

A full-length lily-type lectin (

LTL) was identified from turbot (

Scophthalmus maximus

) in this study. By searching database for protein identification and function prediction

LTL were confirmed. The full-length cDNA of

LTL is composed of 569 bp and contains a 339 bp ORF that encodes 112 amino acid residues. The

LTL peptide is characterized by a specific β-prism architecture and contains three mannose binding sites in a three-fold internal repeat between amino acids 30-99; two of the repeats share the classical mannose binding domain (QxDxNxVxY) while the third binding site was similar to other fish-specific binding motifs (TxTxGxRxV). The primary

secondary

and tertiary structures of

LTL were predicted and analyzed

indicating that the

LTL protein was hydrophilic

contained 5.36%

-helices

39.29% extended strands

16.07% β-folds

and 39.29% random coils

and three β-folds. Quantitative realtime polymerase chain reaction (qPCR) analysis revealed that the

LTL mRNA was abundantly expressed in skin

gill

and intestine. Low levels of

LTL expression were observed in other tissues. The expression of

LTL in gill

skin and intestine increased at mRNA level after stimulation of

Vibrio anguillarum

our results suggest that

LTL serve as the first line of defence against microbial infections and play a pivotal role in the innate mucosal immune system. The current study indicates that

LTL is a member of the lilytype lectin family and the information reported here will provide an important foundation for future research on the role of this protein.

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references

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Molecular characterization and expression analysis of Lilytype lectin (SmLTL) in turbot Scophthalmus maximus, and its response to Vibrio anguillarum

DOI：10.1007/s00343-017-6268-1

摘要

Abstract

关键词

Keywords

references