Effects of dietary supplementation of <italic style="font-style: italic">Ulva pertusa</italic> and nonstarch polysaccharide enzymes on gut microbiota of <italic style="font-style: italic">Siganus canaliculatus</italic>

Xinxu ZHANG; Huijuan WU; Zhongzhen LI; Yuanyou LI; Shuqi WANG; Dashi ZHU; Xiaobo WEN; Shengkang LI

doi:10.1007/s00343-017-6235-x

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Effects of dietary supplementation of Ulva pertusa and nonstarch polysaccharide enzymes on gut microbiota of Siganus canaliculatus

Biology | Updated：2023-05-19

- Effects of dietary supplementation of Ulva pertusa and nonstarch polysaccharide enzymes on gut microbiota of Siganus canaliculatus
- Chinese Journal of Oceanology and Limnology Vol. 36, Issue 2, Pages: 438-449(2018)
- Affiliations：
  
  1.Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China
  2.Marine Biology Institute, Shantou University, Shantou 515063, China
- Author bio：
  
  LI Shengkang, lisk@stu.edu.cn
- Funds：
- DOI：10.1007/s00343-017-6235-x
  CLC：
- Received：19 September 2016，
  
  Accepted：10 November 2016，
  
  Online First：26 December 2016，
  
  Published：2018-03
- Accepted：
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Xinxu ZHANG, Huijuan WU, Zhongzhen LI, et al. Effects of dietary supplementation of Ulva pertusa and nonstarch polysaccharide enzymes on gut microbiota of Siganus canaliculatus[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(2): 438-449.
DOI：

Xinxu ZHANG, Huijuan WU, Zhongzhen LI, et al. Effects of dietary supplementation of Ulva pertusa and nonstarch polysaccharide enzymes on gut microbiota of Siganus canaliculatus[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(2): 438-449. DOI： 10.1007/s00343-017-6235-x.

摘要

Abstract

Fishes represent the highest diversity of vertebrates; however

our understanding of the compositions and functions of their gut microbiota is limited. In this study

we provided the first insight into the gut microbiota of the herbivorous fish

Siganus canaliculatus

by using three molecular ecology techniques based on the 16S rRNA genes (denaturing gradient gel electrophoresis

clone library construction

and highthroughput Illumina sequencing)

and the Illumina sequencing technique is suggested here due to its higher overall coverage of the total 16S rRNA genes. A core gut microbiota of 29 bacterial groups

covering

99.9% of the total bacterial community

was found to be dominated by Proteobacteria and Firmicutes in fish fed three different diets with/without the supplementation of

Ulva pertusa

and non-starch polysaccharide (NSP) enzymes (cellulase

xylanase

and β-glucanase). Diverse potential NSP-degrading bacteria and probiotics (e.g.

Ruminococcus

Clostridium

and Lachnospiraceae) were detected in the intestine of the fish fed

U. pertusa

suggesting that these microorganisms likely participated in the degradation of NSPs derived from

U. pertusa

. This study supports our previous conclusion that

U. pertusa

-based diets are suitable for the production of

S. canaliculatus

with lower costs without compromising quality.

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references

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Effects of dietary supplementation of Ulva pertusa and nonstarch polysaccharide enzymes on gut microbiota of Siganus canaliculatus

DOI：10.1007/s00343-017-6235-x

摘要

Abstract

关键词

Keywords

references