Response of ammonia-oxidizing betaproteobacteria to shortterm fertilization in a salt marsh in China

Yuexin MA; Wei TAO; Jiao LIU; Changfa LIU; Jin LI; Jichen LIU

doi:10.1007/s00343-017-6147-9

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Response of ammonia-oxidizing betaproteobacteria to shortterm fertilization in a salt marsh in China

Ecology | Updated：2023-05-19

- Response of ammonia-oxidizing betaproteobacteria to shortterm fertilization in a salt marsh in China
- Chinese Journal of Oceanology and Limnology Vol. 36, Issue 2, Pages: 351-361(2018)
- Affiliations：
  
  1.Key Laboratory of Mariculture and Stock Enhancement in North China's Sea of Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China
  2.Key Laboratory of Marine Environmental Research of Liaoning Higher Education, Dalian 116023, China
  3.Institute of Ocean and Fisheries of Panjin, Panjin 124001, China
- Author bio：
  
  MA Yuexin, mayuexin@dlou.edu.cn
- Funds：
- DOI：10.1007/s00343-017-6147-9
  CLC：
- Received：26 May 2016，
  
  Accepted：19 August 2016，
  
  Online First：26 December 2016，
  
  Published：2018-02
- Accepted：
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Yuexin MA, Wei TAO, Jiao LIU, et al. Response of ammonia-oxidizing betaproteobacteria to shortterm fertilization in a salt marsh in China[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(2): 351-361.
DOI：

Yuexin MA, Wei TAO, Jiao LIU, et al. Response of ammonia-oxidizing betaproteobacteria to shortterm fertilization in a salt marsh in China[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(2): 351-361. DOI： 10.1007/s00343-017-6147-9.

摘要

Abstract

This study examines the impacts of short-term (6 months) fertilization on the community structure and abundance of ammonia-oxidizing betaproteobacteria (β-AOB) and the potential nitrification rate in sediment colonized by

Suaeda heteroptera

in a saltmarsh located in Shuangtai estuary

China. The sediment samples were collected from plots treated with different amounts of an N fertilizer (urea supplied at 0.1

0.2

0.4

and 0.8 g/kg (nitrogen content in dry sediment))

and with different forms of N fertilizers (urea

(NH

)

and NH

each supplied at 0.2 g/kg). The fertilizers were applied 1-4 times during the plant-growing season in May

July

August and September of 2013. Untreated plots were included as a control. As revealed in denaturing gradient gel electrophoresis of the 16S rRNA gene

the β-AOB community responded to both the amount and form of N. Real-time quantitative PCR indicated that both abundance and potential nitrification rate of β-AOB increased after N addition

regardless of concentration and form (except NH

). These results provide evidence that short-term N application influences the sediment β-AOB community

β-AOB abundance and potential nitrification rate in a saltmarsh ecosystem.

关键词

Keywords

references

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