Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage

Xie Shijun; Li Fuhua; Zhang Xiaojun; Zhang Jiquan; Xiang Jianhai

doi:10.1007/s00343-017-6109-2

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Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage

Updated：2023-05-16

- Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage
- Journal of Oceanology and Limnology Vol. 35, Issue 6, Pages: 1524-1530(2017)
- Affiliations：
  
  1. Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
  3. Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology
- Author bio：
- Funds：
- DOI：10.1007/s00343-017-6109-2
  CLC：
- Online First：13 May 2017，
  
  Published：2017
- Accepted：
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Xie, S., Li, F., Zhang, X. et al. Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage., Chin. J. Ocean. Limnol. 35, 1524–1530 (2017). https://doi.org/10.1007/s00343-017-6109-2
DOI：

Xie, S., Li, F., Zhang, X. et al. Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage., Chin. J. Ocean. Limnol. 35, 1524–1530 (2017). https://doi.org/10.1007/s00343-017-6109-2 DOI：

摘要

Abstract

The peritrophic membrane plays an important role in the defense system of the arthropod gut. The digestive tract is considered one of the major tissues targeted by white spot syndrome virus (WSSV) in shrimp. In this study

the nucleotide sequence encoding peritrophin-like protein of Litopenaeus vannamei (LvPT) was amplified from a yeast two-hybrid library of L. vannamei. The epitope peptide of LvPT was predicted with the GenScript OptimumAntigen™ design tool. An anti-LvPT polyclonal antibody was produced and shown to specifically bind a band at ~27 kDa

identified as LvPT. The LvPT protein was expressed and its concentration determined. LvPT dsRNA (4 μg per shrimp) was used to inhibit LvPT expression in shrimp

and a WSSV challenge experiment was then performed with reverse gavage. The pleopods

stomachs

and guts were collected from the shrimp at 0

and 72 h post-infection (hpi). Viral load quantification showed that the levels of WSSV were significantly lower in the pleopods

stomachs

and guts of shrimp after LvPT dsRNA interference than in those of the controls at 48 and 72 hpi. Our results imply that LvPT plays an important role during WSSV infection of the digestive tract.

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references

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