Molecular characterization and expression profiles of four transformer-2 isoforms in the Chinese mitten crab Eriocheir sinensis

Danli Luo; Yuan Liu; Min Hui; Chengwen Song; Hourong Liu; Zhaoxia Cui

doi:10.1007/s00343-017-6071-z

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Molecular characterization and expression profiles of four transformer-2 isoforms in the Chinese mitten crab Eriocheir sinensis

Updated：2023-05-16

- Molecular characterization and expression profiles of four transformer-2 isoforms in the Chinese mitten crab Eriocheir sinensis
- Journal of Oceanology and Limnology Vol. 35, Issue 4, Pages: 782-791(2017)
- Affiliations：
  
  1. Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
  3. Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology
  4. National & Local Joint Engineering Laboratory of Ecological Mariculture
- Author bio：
- Funds：
- DOI：10.1007/s00343-017-6071-z
  CLC：
- Online First：08 September 2016，
  
  Published：2017
- Accepted：
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Luo, D., Liu, Y., Hui, M. et al. Molecular characterization and expression profiles of four transformer-2 isoforms in the Chinese mitten crab Eriocheir sinensis, ., Chin. J. Ocean. Limnol. 35, 782–791 (2017). https://doi.org/10.1007/s00343-017-6071-z
DOI：

Luo, D., Liu, Y., Hui, M. et al. Molecular characterization and expression profiles of four transformer-2 isoforms in the Chinese mitten crab Eriocheir sinensis, ., Chin. J. Ocean. Limnol. 35, 782–791 (2017). https://doi.org/10.1007/s00343-017-6071-z DOI：

摘要

Abstract

The transformer-2 (tra-2) gene plays a key role in the regulatory hierarchy of sexual differentiation in somatic tissues and in the germline of Drosophila melanogaster. In this study

sequences and expression profiles of tra-2 in the Chinese mitten crab Eriocheir sinensis were characterized. Four tra-2 isoforms

designated as Estra-2a

Estra-2b

Estra-2c

and Estra-2d

were isolated. They all contained an RNA-recognition motif (RRM) and a linker region

which shared high similarity with other reported tra-2s. Sequence analysis revealed that Estra-2a

Estra-2b and Estra-2c are encoded by the same genomic locus and are generated by alternative splicing of the pre-mRNA. Compared with the other three isoforms

Estra-2d lacks the RS2 domain. Quantitative real-time PCR showed that all four isoforms were highly expressed in the fertilized egg

and in the 2–4 cell and blastula stages compared with larval stages (P≤0.01)

suggesting their maternal origin in early embryonic developmental stages. Notably

Estra-2a was highly expressed in male somatic tissues

while Estra-2c was significantly highly expressed in the ovary. These results suggest that Estra-2c is involved in sexual differentiation of the Chinese mitten crab. Our findings provide basic information for further functional studies of the tra-2 gene/protein in this species.

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