Improvement of Nannochloropsis oceanica growth performance through chemical mutation and characterization of fast growth physiology by transcriptome profiling

Sijie Liang; Li Guo; Genmei Lin; Zhongyi Zhang; Haiyan Ding; Yamei Wang; Guanpin Yang

doi:10.1007/s00343-017-6023-7

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Improvement of Nannochloropsis oceanica growth performance through chemical mutation and characterization of fast growth physiology by transcriptome profiling

Updated：2023-05-16

- Improvement of Nannochloropsis oceanica growth performance through chemical mutation and characterization of fast growth physiology by transcriptome profiling
- Journal of Oceanology and Limnology Vol. 35, Issue 4, Pages: 792-802(2017)
- Affiliations：
  
  1. Laboratory of Marine Genetics and Breeding Ocean University of China
  2. College of Marine Life Sciences Ocean University of China
  3. Institutes of Evolution and Marine Biodiversity Ocean University of China
- Author bio：
- Funds：
- DOI：10.1007/s00343-017-6023-7
  CLC：
- Online First：08 September 2016，
  
  Published：2017
- Accepted：
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Liang, S., Guo, L., Lin, G. et al. Improvement of Nannochloropsis oceanica growth performance through chemical mutation and characterization of fast growth physiology by transcriptome profiling., Chin. J. Ocean. Limnol. 35, 792–802 (2017). https://doi.org/10.1007/s00343-017-6023-7
DOI：

Liang, S., Guo, L., Lin, G. et al. Improvement of Nannochloropsis oceanica growth performance through chemical mutation and characterization of fast growth physiology by transcriptome profiling., Chin. J. Ocean. Limnol. 35, 792–802 (2017). https://doi.org/10.1007/s00343-017-6023-7 DOI：

摘要

Abstract

Nannochloropsis oceanica promises to be an industrial-level producer of polyunsaturated fatty acids. In this study

the fastest and slowest growing N. oceanica mutants were selected through N-methyl-N’-nitro-N-nitrosoguanidine mutation

and two mutant strains and the wild type (WT) subjected to transcriptome profiling. It was found that the OD680 reads at stationary growth phase of both WT and its mutants were proportional to their cell density

thus indicating their division rate and growth speed during culture. This chemical mutation was effective for improving growth performance

and the fast strain divided faster by upregulating the expression of genes functioning in the cell cycle and downregulating genes involved in synthesis of amino acids

fatty acids

and sugars as well as the construction of ribosome and photosynthetic machinery. However

the relationship among the effected genes responsible for cell cycle

metabolism of fatty and amino acids

and construction of ribosome and photosynthetic machinery remained unclear. Further genetic studies are required for clarifying the genetic/metabolic networks underpinning the growth performance of N. oceanica. These findings demonstrated that this mutation strategy was effective for improving the growth performance of this species and explored a means of microalgal genetic improvement

particularly in species possessing a monoploid nucleus and asexual reproduction.

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Keywords

references

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