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Recovery of gonadal development in tiger puffer Takifugu rubripes after exposure to 17β-estradiol during early life stages
- Journal of Oceanology and Limnology Vol. 35, Issue 3, Pages: 613-623(2017)
- Affiliations：
1. Key Laboratory of Mariculture of Ministry of Education Ocean University of China
2. Municipal Key Laboratory of Marine Fish Breeding and Biotechnology Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
3. Tianyuan Aquaculture Co. Ltd. of Yantai Economic Development Zone
- Author bio：
- Funds：
DOI：10.1007/s00343-017-6016-6
CLC：-
Online First：14 June 2016，
Published：2017
- Accepted：
Scan QR Code
Hu, P., Liu, B., Meng, Z. et al. Recovery of gonadal development in tiger puffer Takifugu rubripes after exposure to 17β-estradiol during early life stages., Chin. J. Ocean. Limnol. 35, 613–623 (2017). https://doi.org/10.1007/s00343-017-6016-6
摘要
Abstract
The aim of the present study was to investigate the long-term effects of 17β-estradiol (E2) exposure on gonadal development in the tiger puff er (Takifugu rubripes)
which has a genetic sex determination system of male homogametic XY-XX. Tiger puff er larvae were exposed to 1
10 and 100 μg/L E2 from 15 to 100 days post-hatch (dph) and then maintained in clean seawater until 400 dph. Changes in sex ratio
gonadal structure and gonadosomatic index (GSI) were monitored at 100
160
270 and 400 dph. Sex-associated single nucleotide polymorphism (SNP) markers were used to analyze the genetic sex of samples
except those at 100 dph. Exposure had a positive effect on the conversion of genetically male gonads into phenotypically female gonads at 100 dph. However
gonads from 60% of genetic XY males in the 1-μg/L E2 group and 100% in the 10-μg/L E2 group developed intersexual gonads at 160 dph; gonads of all genetic XY males in the two treatment groups reverted to testis by 270 dph. While 38%
57% and 44% of gonads of XY fish in the 100-μg/L E2 group reverted to intersexual gonads at 160
270 and 400 dph
respectively
none reverted to testis after E2 treatment. In addition
E2 exposure inhibited gonadal growth of both genetic sexes
as indicated by the clear dose-dependent decrease in GSI at 270 and 400 dph. The results showed that exposure to E2 during the early life stages of tiger puff er disrupted gonadal development
but that fish recovered after migration to clean seawater. The study suggests the potential use of tiger puff er as a valuable indicator species to evaluate the effects of environmental estrogens on marine fish
thereby protecting valuable fishery resources.
关键词
Keywords
references
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