Effects of Lugol’s iodine solution and formalin on cell volume of three bloom-forming dinoflagellates

Yang Yang; Xiaoxia Sun; Yongfang Zhao

doi:10.1007/s00343-017-5378-0

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Effects of Lugol’s iodine solution and formalin on cell volume of three bloom-forming dinoflagellates

Updated：2023-05-16

- Effects of Lugol’s iodine solution and formalin on cell volume of three bloom-forming dinoflagellates
- Journal of Oceanology and Limnology Vol. 35, Issue 4, Pages: 858-866(2017)
- Affiliations：
  
  1. Jiaozhou Bay Marine Ecosystem Research Station Institute of Oceanology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
  3. Laboratory for Marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and Technology
- Author bio：
- Funds：
- DOI：10.1007/s00343-017-5378-0
  CLC：
- Online First：08 September 2016，
  
  Published：2017
- Accepted：
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Yang, Y., Sun, X. & Zhao, Y. Effects of Lugol’s iodine solution and formalin on cell volume of three bloom-forming dinoflagellates., Chin. J. Ocean. Limnol. 35, 858–866 (2017). https://doi.org/10.1007/s00343-017-5378-0
DOI：

Yang, Y., Sun, X. & Zhao, Y. Effects of Lugol’s iodine solution and formalin on cell volume of three bloom-forming dinoflagellates., Chin. J. Ocean. Limnol. 35, 858–866 (2017). https://doi.org/10.1007/s00343-017-5378-0 DOI：

摘要

Abstract

Fixatives are traditionally used in marine ecosystem research. The bias introduced by fixatives on the dimensions of plankton cells may lead to an overestimation or underestimation of the carbon biomass. To determine the impact of traditional fixatives on dinoflagellates during short- and long-term fixation

we analyzed the degree of change in three bloom-forming dinoflagellates (Prorocentrum micans

Scrippsiella trochoidea and Noctiluca scintillans) brought about by Lugol’s iodine solution (hereafter Lugol’s) and formalin. The fixation effects were species-specific. P. micans cell volume showed no significant change following long-term preservation

and S. trochoidea swelled by approximately 8.06% in Lugol’s and by 20.97% in formalin as a percentage of the live cell volume

respectively. N. scintillans shrank significantly in both fixatives. The volume change due to formalin in N. scintillans was not concentration-dependent

whereas the volume shrinkage of N. scintillans cells fixed with Lugol’s at a concentration of 2% was nearly six-fold that in cells fixed with Lugol’s at a concentration of 0.6%–0.8%. To better estimate the volume of N. scintillans fixed in formalin at a concentration of 5%

we suggest that the conversion relationship was as follows: volume of live cell=volume of intact fixed cell/0.61. Apart from size change

damage induced by fixatives on N. scintillans was obvious. Lugol’s is not a suitable fixative for N. scintillans due to high frequency of broken cells. Accurate carbon biomass estimate of N. scintillans should be performed on live samples. These findings help to improve the estimate of phytoplankton cell volume and carbon biomass in marine ecosystem.

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references

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