Inbreeding and genetic diversity analysis in a hatchery release population and clones of Rhopilema esculentum based on microsatellite markers

Tao Tian; Zaizhong Chen; Mosang Wang; Yulong Hu; Weiji Wang

doi:10.1007/s00343-017-5333-0

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Inbreeding and genetic diversity analysis in a hatchery release population and clones of Rhopilema esculentum based on microsatellite markers

Updated：2023-05-16

- Inbreeding and genetic diversity analysis in a hatchery release population and clones of Rhopilema esculentum based on microsatellite markers
- Journal of Oceanology and Limnology Vol. 35, Issue 3, Pages: 580-586(2017)
- Affiliations：
  
  1. College of Fisheries and Life Science Shanghai Ocean University
  2. Key Laboratory of Sustainable Development of Marine Fisheries Ministry of Agriculture Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
  3. Function Laboratory for Marine Fisheries Science and Food Production Processes National Laboratory for Ocean Science and Technology
- Author bio：
- Funds：
- DOI：10.1007/s00343-017-5333-0
  CLC：
- Online First：09 July 2016，
  
  Published：2017
- Accepted：
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Tian, T., Chen, Z., Wang, M. et al. Inbreeding and genetic diversity analysis in a hatchery release population and clones of Rhopilema esculentum based on microsatellite markers., Chin. J. Ocean. Limnol. 35, 580–586 (2017). https://doi.org/10.1007/s00343-017-5333-0
DOI：

Tian, T., Chen, Z., Wang, M. et al. Inbreeding and genetic diversity analysis in a hatchery release population and clones of Rhopilema esculentum based on microsatellite markers., Chin. J. Ocean. Limnol. 35, 580–586 (2017). https://doi.org/10.1007/s00343-017-5333-0 DOI：

摘要

Abstract

Ten microsatellite markers were used to analyze the levels of genetic diversity and inbreeding in a hatchery release population of Rhopilema esculentum Kishinouye (Scyphozoa: Rhizostomatidae). A total of 85 alleles were detected in 600 individuals. Within-population levels of observed (H o) and expected (H e) heterozygosity ranged from 0.152 to 0.839 (mean=0.464) and from 0.235 to 0.821 (mean=0.618)

respectively. The polymorphism information content (PIC) of each marker ranged from 0.207 to 0.795 with an average of 0.580

indicating that the hatchery population maintained a high level of genetic diversity. Inbreeding levels were estimated in the hatchery population and the inbreeding coefficient was 0.203. This result revealed that a certain level of inbreeding occurred within the population. Meanwhile

we also determined genetic diversity at the clone level. Several polyps from the same scyphistomae were genotyped at the ten microsatellite loci and there was virtually no difference in their genotypes. Furthermore

we calculated the probabilities of exclusion. When both parents were known

the average exclusion probability of ten loci was 99.99%. Our data suggest that the ten microsatellite markers can not only be used to analyze the identity of individuals but they can also be applied to parentage identification. Our research provides a theoretical basis and technical support for genetic diversity detection and reasonable selection of R. esculentum hatchery populations. These findings support the use of releasing studies and conservation of R. esculentum germplasm resources.

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references

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