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Intestinal disease of scattered mirror carp Cyprinus carpio caused by Thelohanellus kitauei and notes on the morphology and phylogeny of the myxosporean from Sichuan Province, southwest China
- Journal of Oceanology and Limnology Vol. 35, Issue 3, Pages: 587-596(2017)
- Affiliations：
1. Key Laboratory of Fishery Ecology and Environment Guangdong Province
2. Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization Ministry of Agriculture
3. South China Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
4. Key Laboratory of Aquatic Biodiversity and Conservation and the Institute of Hydrobiology Chinese Academy of Sciences
5. Sichuan Fisheries School
- Author bio：
- Funds：
DOI：10.1007/s00343-017-5312-5
CLC：-
Online First：26 May 2016，
Published：2017
- Accepted：
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Ye, L., Lu, M., Quan, K. et al. Intestinal disease of scattered mirror carp Cyprinus carpio caused by Thelohanellus kitauei and notes on the morphology and phylogeny of the myxosporean from Sichuan Province, southwest China., Chin. J. Ocean. Limnol. 35, 587–596 (2017). https://doi.org/10.1007/s00343-017-5312-5
Ye, L., Lu, M., Quan, K. et al. Intestinal disease of scattered mirror carp Cyprinus carpio caused by Thelohanellus kitauei and notes on the morphology and phylogeny of the myxosporean from Sichuan Province, southwest China., Chin. J. Ocean. Limnol. 35, 587–596 (2017). https://doi.org/10.1007/s00343-017-5312-5 DOI：
摘要
Abstract
The mass mortality of pond-reared scattered mirror carp
Cyprinus carpio
caused by Thelohanellus kitauei
occurred at fish farms in Sichuan Province
southwest China. Morphological and molecular analyses were supplemented with histological evaluation of infected tissues to better understand the route of infection and the pathological effects of T. kitauei on the fish host. The intestine of the diseased host was full of large cysts of the myxosporean. The cysts range from 2 cm to 3.6 cm in diameter. Histopathology indicated that T. kitaue i first invaded the submucosa of the host intestine and then moved into the mucosa layers with the development of their spores
finally entering into the enteric cavity of the hosts after the disruption of mucosa layers. The pyriform spores of T. kitauei were surrounded by the transparent spore sheath
measuring 25.98 μm±0.95 μm in body length
8.72 μm±0.51 μm in body width
and 7.86 μm±0.26 μm in body thickness. The single polar capsule was pyriform
measuring 14.73 μm±0.92 μm in length and 6.82 μm±0.45 μm in width
with eight to 10 turns of filament coils winding inside. Phylogenetic analysis based on the 18S small-subunit ribosomal DNA sequences indicated that minimal genetic differences were present between T. kitauei samples from South Korea and from China. Close affinity was found between the genus Thelohanellus and Myxobolus. Additionally
two polar capsule nuclei were found at the anterior end of the single polar capsule in spores of T. kitauei stained with hematoxylin and eosin
which suggested the separation of the genus Thelohanellus from Myxobolus.
关键词
Keywords
references
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