Construction of a growth model in the green alga Tetraselmis subcordiformis using a response surface approach

Hui Wang; Jingyan Niu; Jiahui Liu; Hongshuai Yang; Zhigang Liu

doi:10.1007/s00343-017-5298-z

Your Location：

Home >

Browse articles >

Construction of a growth model in the green alga Tetraselmis subcordiformis using a response surface approach

Updated：2023-05-16

- Construction of a growth model in the green alga Tetraselmis subcordiformis using a response surface approach
- Journal of Oceanology and Limnology Vol. 35, Issue 3, Pages: 537-545(2017)
- Affiliations：
  
  1. Life Sciences College Huaiyin Normal University
  2. Polytechnic Department Jiaozuo Teachers College
  3. Fisheries College Guangdong Ocean University
- Author bio：
- Funds：
- DOI：10.1007/s00343-017-5298-z
  CLC：
- Online First：29 June 2016，
  
  Published：2017
- Accepted：
Scan QR Code
Wang, H., Niu, J., Liu, J. et al. Construction of a growth model in the green alga Tetraselmis subcordiformis using a response surface approach., Chin. J. Ocean. Limnol. 35, 537–545 (2017). https://doi.org/10.1007/s00343-017-5298-z
DOI：

Wang, H., Niu, J., Liu, J. et al. Construction of a growth model in the green alga Tetraselmis subcordiformis using a response surface approach., Chin. J. Ocean. Limnol. 35, 537–545 (2017). https://doi.org/10.1007/s00343-017-5298-z DOI：

摘要

Abstract

The green alga Tetraselmis subcordiformis has been widely used as a quality live food for aquaculture species

and also has been studied as a model organism for the photo-biological production of hydrogen. We attempted to quantify the relationship between T. subcordiformis specific growth rate (SGR) and three important environmental factors (temperature

salinity

and pH) using the central composite design and response surface method under laboratory conditions. The results showed that the linear effects of temperature and salinity were significant (P< 0.05)

and they were equally important in impacting T. subcordiformis specific growth; the linear effect of pH was not significant (P>0.05); the interactive effect of temperature and pH was significant (P<0.05)

whereas the temperature × salinity and salinity × pH interactions were not significant (P>0.05); all of the quadratic effects of the three factors were significant (P<0.05). A model equation for specific growth rate with the three factors was established

with the unadjusted and predictive R 2 as high as 0.990 and 0.921

respectively

suggesting that the model was a very good fit and that it could be used to predict SGR. Through optimizing the reliable model

an optimal 3-factor combination of 25 °C/35 of salinity/pH 7.9 was obtained

at which the maximum specific growth rate (0.65) was recorded

with a desirability value of 93.8%. These experimental results could serve as guidelines for increasing T. subcordiformis production efficiency.

关键词

Keywords

references

Andersen R A. 2005. Algal Culturing Techniques. Academic Press, San Diego, CA, US. p.51–85.

Becker E W. 2008. Microalgae: Biotechnology and Microbiology. Cambridge University Press, Cambridge. p.56–62.

Cai Z P, Duan S S, Zhu H H. 2012. Optical density method and cell count method for determining the growth of three energy microalgae and their correlation and verification. J. South. Agric., 43 (10): 480–1484.

Cho S H, Ji S C, Hur S B, Bae J, Park I S, Song Y C. 2007. Optimum temperature and salinity conditions for growth of green algae Chlorella ellipsoidea and Nannochloris oculata. Fish. Sci., 73 (5): 050–1056.

Davis T W, Berry D L, Boyer G L, Gobler C J. 2009. The effects of temperature and nutrients on the growth and dynamics of toxic and non-toxic strains of Microcystis during cyanobacteria blooms. Harmful Algae, 8 (5): 15–725.

Davison I R. 1991. Environmental effects on algal photosynthesis: temperature. J. Phycol., 27 (1): 2–8.

Day J D, Edwards A P, Rodgers G A. 1991. Development of an industrial-scale process for the heterotrophic production of a micro-algal mollusc feed. Bioresour. Technol., 38 (2-3): 245–249.

Guan Y F, Deng M C, Yu X J, Zhang W. 2004a. Two-stage photo-biological production of hydrogen by marine green alga Platymonas subcordiformis. Biochem. Eng. J., 19 (1): 9–73.

Guan Y F, Zhang W, Deng M C, Jin M F, Yu X J. 2004b. Significant enhancement of photobiological H 2 evolution by carbonylcyanide m -chlorophenylhydrazone in the marine green alga Platymonas subcordiformis. Biotechnol. Lett., 26 (13): 031–1035.

Guillard R R L, Ryther J H. 1962. Studies of marine planktonic diatoms. I. Cyclotella nana hustedt, and Detonula confervacea (Cleve) gran. Can. J. Microbiol., 8 (2): 29–239.

Guo Z, Chen Z A, Zhang W, Yu X J, Jin M F. 2008. Improved hydrogen photoproduction regulated by carbonylcyanide m -chlorophenylhrazone from marine green alga Platymonas subcordiformis grown in CO2-supplemented air bubble column bioreactor. Biotechnol. Lett., 30 (5): 77–883.

Huang X X, Huang Z Z, Wen W, Yan J Q. 2013. effects of nitrogen supplementation of the culture medium on the growth, total lipid content and fatty acid profiles of three microalgae (Tetraselmis subcordiformis, Nannochloropsis oculata and Pavlova viridis). J. Appl. Phycol., 25 (1): 29–137.

Huang Z Z, Huang X X, Yan J Q, Zhu J. 2011. effects of growth phase and temperature on the total ATPase activity of microalgae. Mar. Fish., 33 (2): 81–186. (in Chinese with English abstract)

Ji C F, Yu X J, Chen Z A, Xue S, Legrand J, Zhang W. 2011. effects of nutrient deprivation on biochemical compositions and photo-hydrogen production of Tetraselmis subcordiformis. Int. J. Hydrogen Energy, 36 (10): 817–5821.

Kirst G O. 1977a. Ion composition of unicellular marine and fresh-water algae, with special reference to Platymonas subcordiformis cultivated in media with different osmotic strengths. Oecologia, 28 (2): 77–189.

Kirst G O. 1977b. Coordination of ionic relations and mannitol concentrations in the euryhaline unicellular alga, Platymonas subcordiformis (Hazen) after osmotic shocks. Planta, 135 (1): 9–75.

Kosourov S, Seibert M, Ghirardi M L. 2003. effects of extracellular pH on the metabolic pathways in sulfurdeprived, H 2 -producing Chlamydomonas Reinhardtii cultures. Plant Cell Physiol., 44 (2): 46–155.

Leland H V, Brown L R, Mueller D K. 2001. Distribution of algae in the San Joaquin River, California, in relation to nutrient supply, salinity and other environmental factors. Freshw. Biol., 46 (9): 139–1167.

Li F, Ge C Z, Fang J G, Mao Y Z, Yu S T. 2007. A preliminary study on multiplication of Platymonas subcordiformis at different temperatures and inoculation densities. Mar. Fish. Res., 28 (6): 1–66. (in Chinese with English abstract)

Liu J H, Yang H S, Wang H. 2014. Constructing the relationship of growth of Tetraselmis subcordiformis with temperature and salinity. J. Guangdong Ocean Univ., 34 (1): 0–65. (in Chinese with English abstract)

Liu Y, Chen Z A, Lu H B, Liu C F, Jin M F, Guo Z, Zhang W. 2007. Optimization of culture medium and photosynthetic characteristics of Platymonas subcordiformis. Chin. J. Process Eng., 7 (6): 197–1201. (in Chinese with English abstract)

Montgomery D C. 2005. Design and Analysis of Experiments. Wiley, New York. p.384–418.

Moss B. 1973. The influence of environmental factors on the distribution of freshwater algae: an experimental study: II. The role of pH and the carbon dioxide-bicarbonate system. J. Ecol., 61 (1): 57–177.

Naoki S, Norio M, Yoshiro M, Nobuo U. 1979. effect of growth temperature on lipid and fatty acid compositions in the blue-green algae, Anabaena variabilis and Anacystis nidulans. Biochim. Biophys. Acta -Lipids Lipid Metabol., 572 (1): 9–28.

Pang S J, Zhang Z H, Bao Y, Gao S Q. 2006. Settling abalone veliger larvae in a free-swimming microalgal culture. Aquaculture, 258 (1-4): 327–336.

Raven J A, Geider R J. 1988. Temperature and algal growth. New Phytol., 110 (4): 41–461.

Satoh A, Kurano N, Miyachi S. 2001. Inhibition of photosynthesis by intracellular carbonic anhydrase in microalgae under excess concentrations of CO2. Photosynth. Res., 68 (3): 15–224.

Su J Q, Zheng T L, Hu Z, Xu J S, Yu Z M, Song X X. 2003. effects of marine bacteria on the growth and toxin production of red-tide algae under different pH and salinities. Chin. J. Appl. Ecol., 14 (7): 161–1164. (in Chinese with English abstract)

Sudhir P, Murthy S D S. 2004. effects of salt stress on basic processes of photosynthesis. Photosynthetica, 42 (4): 81–486.

Tang B J, Liu B Z, Wang G D, Zhang T, Xiang J H. 2006. effects of various algal diets and starvation on larval growth and survival of Meretrix meretrix. Aquaculture, 254 (1-4): 526–533.

Taylor R, Fletcher R L, Raven J A. 2001. Preliminary studies on the growth of selected ‘green tide’ algae in laboratory culture: effects of irradiance, temperature, salinity and nutrients on growth rate. Bot. Mar., 44 (4): 27–336.

Xu H, Liu Z P, Yuan L, Yang L Z. 2009. effect of pH on growth of several freshwater algae. Environ. Sci. Technol., 32 (1): 7–30. (in Chinese with English abstract)

Yan F, Chen Z A, Li W, Cao X P, Xue S, Zhang W. 2011. Purification and characterization of a hydrogenase from the marine green alga Tetraselmis subcordiformis. Process Biochem., 46 (5): 212–1215.

Yao C H, Ai J N, Cao X P, Xue S, Zhang W. 2012. Enhancing starch production of a marine green microalga Tetraselmis subcordiformis through nutrient limitation. Bioresour. Technol., 118: 38–444.

Yao C H, Ai J N, Cao X P, Xue S. 2013. Salinity manipulation as an effective method for enhanced starch production in the marine microalga Tetraselmis subcordiformis. Bioresour. Technol., 146: 63–671.

Zhou H Q, Yi C P, Ding Z P, Zhang J P, Miao H. 2001. effects of environmental factors on fatty acid biosynthesis of Platymonas helgolandica var Tsing t aoensis, Platymonas subcordiformis and Nannochloris oculata. J. Zhejiang Ocean Univ. (Nat. Sci.), 20 (S1): 112–117. (in Chinese with English abstract)

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Grey relational analysis on the relation between marine environmental factors and oxidation-reduction potential

Sound speed profiles in high spatiotemporal resolution using multigrid three-dimensional variational method: a coastal experiment off northern Shandong Peninsula

Upper ocean response to typhoon Kujira (2015) in the South China Sea by multiple means of observation

Effects of temperature and salinity on the asexual reproduction of Aurelia coerulea polyps

Effect of temperature, salinity, and body length on the energy budget of Daphniopsis tibetana Sars (Cladocera: Daphniidae)

Related Author

Xueqing Liu

Jia Wang

Dun Zhang

Yantao Li

Guangchao HOU

Jingsheng ZHAI

Qi SHAO

Yanling ZHAO

Related Institution

Marine Environmental Engineering Center Institute of Oceanology Chinese Academy of Sciences

College of Chemistry and Chemical Engineering Ocean University of China

School of Marine Science and Technology, Tianjin University

Tianjin Key Laboratory for Oceanic Meteorology

The PLA 31010 Units

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰