Polymorphisms of clip domain serine proteinase and serine proteinase homolog in the swimming crab Portunus trituberculatus and their association with Vibrio alginolyticus

Meng Liu; Yuan Liu; Min Hui; Chengwen Song; Zhaoxia Cui

doi:10.1007/s00343-016-5331-7

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Polymorphisms of clip domain serine proteinase and serine proteinase homolog in the swimming crab Portunus trituberculatus and their association with Vibrio alginolyticus

Updated：2023-05-16

- Polymorphisms of clip domain serine proteinase and serine proteinase homolog in the swimming crab Portunus trituberculatus and their association with Vibrio alginolyticus
- Journal of Oceanology and Limnology Vol. 35, Issue 2, Pages: 235-243(2017)
- Affiliations：
  
  1. College of Fisheries and Life Science Dalian Ocean University
  2. Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences
  3. Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology
  4. National & Local Joint Engineering Laboratory of Ecological Mariculture
- Author bio：
- Funds：
- DOI：10.1007/s00343-016-5331-7
  CLC：
- Online First：26 May 2016，
  
  Published：2017
- Accepted：
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Liu, M., Liu, Y., Hui, M. et al. Polymorphisms of clip domain serine proteinase and serine proteinase homolog in the swimming crab Portunus trituberculatus and their association with Vibrio alginolyticus, ., Chin. J. Ocean. Limnol. 35, 235–243 (2017). https://doi.org/10.1007/s00343-016-5331-7
DOI：

Liu, M., Liu, Y., Hui, M. et al. Polymorphisms of clip domain serine proteinase and serine proteinase homolog in the swimming crab Portunus trituberculatus and their association with Vibrio alginolyticus, ., Chin. J. Ocean. Limnol. 35, 235–243 (2017). https://doi.org/10.1007/s00343-016-5331-7 DOI：

摘要

Abstract

Clip domain serine proteases (cSPs) and their homologs (SPHs) play an important role in various biological processes that are essential components of extracellular signaling cascades

especially in the innate immune responses of invertebrates. Here

polymorphisms of PtcSP and PtSPH from the swimming crab Portunus trituberculatus were investigated to explore their association with resistance/susceptibility to Vibrio alginolyticus. Polymorphic loci were identified using Clustal X

and characterized with SPSS 16.0 software

and then the significance of genotype and allele frequencies between resistant and susceptible stocks was determined by a χ 2 test. A total of 109 and 77 single nucleotide polymorphisms (SNPs) were identified in the genomic fragments of PtcSP and PtSPH

respectively. Notably

nearly half of PtSPH polymorphisms were found in the non-coding exon 1. Fourteen SNPs investigated were significantly associated with susceptibility/resistance to V. alginolyticus (P <0.05). Among them

eight SNPs were observed in introns

and one synonymous

four non-synonymous SNPs and one ins-del were found in coding exons. In addition

five simple sequence repeats (SSRs) were detected in intron 3 of PtcSP. Although there was no statistically significant difference of allele frequencies

the SSRs showed different polymorphic alleles on the basis of the repeat number between resistant and susceptible stocks. After further validation

polymorphisms investigated here might be applied to select potential molecular markers of P. trituberculatus with resistance to V. alginolyticus.

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references

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