Measurement and feature analysis of absorption spectra of four algal species

Jianhua Zhu; Hongli Zhou; Bing Han; Tongji Li

doi:10.1007/s00343-016-5321-9

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Measurement and feature analysis of absorption spectra of four algal species

Updated：2023-05-16

- Measurement and feature analysis of absorption spectra of four algal species
- Journal of Oceanology and Limnology Vol. 35, Issue 2, Pages: 350-359(2017)
- Affiliations：
  
  National Ocean Technology Center
- Author bio：
- Funds：
- DOI：10.1007/s00343-016-5321-9
  CLC：
- Online First：26 April 2016，
  
  Published：2017
- Accepted：
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Zhu, J., Zhou, H., Han, B. et al. Measurement and feature analysis of absorption spectra of four algal species., Chin. J. Ocean. Limnol. 35, 350–359 (2017). https://doi.org/10.1007/s00343-016-5321-9
DOI：

Zhu, J., Zhou, H., Han, B. et al. Measurement and feature analysis of absorption spectra of four algal species., Chin. J. Ocean. Limnol. 35, 350–359 (2017). https://doi.org/10.1007/s00343-016-5321-9 DOI：

摘要

Abstract

Two methods for particulate pigments (i.e.

quantitative filter technique

QFT

and in vivo measurement

InVivo

respectively) and two methods for dissolved pigments (i.e.

Acetone Extracts

AceEx

and high-performance liquid chromatography

HPLC

respectively) were used to obtain the optical absorption coefficient spectra for cultures of four typical algal species. Through normalization and analysis of the spectra

it is shown that (1) the four methods are able to measure optical absorption spectra of particulate and/or dissolved pigments; (2) that the optical absorption spectra of particulate and dissolved pigments were consistent in terms of the peak position in the blue wavelength

and the difference of the peak position in the near infrared wavelength was ~10 nm between each other; and (3) that the leveling effect of the absorption spectra of particulate pigments was significant. These four methods can all effectively measure the absorption coefficients of phytoplankton pigments

while each one has its unique advantages in different applications. Therefore

appropriate method should be carefully selected for various application due to their intrinsic difference.

关键词

Keywords

references

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