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The combined effects of Dolichospermum flos-aquae, light, and temperature on microcystin production by Microcystis aeruginosa
- Journal of Oceanology and Limnology Vol. 34, Issue 6, Pages: 1173-1182(2016)
- Affiliations：
State Key Laboratory of Pharmaceutical Biotechnology School of Life Science Nanjing University
- Author bio：
- Funds：
DOI：10.1007/s00343-016-5204-0
CLC：-
Online First：14 March 2016，
Published：2016
- Accepted：
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Chen, R., Li, F., Liu, J. et al. The combined effects of Dolichospermum flos-aquae, light, and temperature on microcystin production by Microcystis aeruginosa, ., Chin. J. Ocean. Limnol. 34, 1173–1182 (2016). https://doi.org/10.1007/s00343-016-5204-0
摘要
Abstract
The effects of light
temperature
and coculture on the intracellular microcystin-LR (MC-LR) quota of Microcystis aeruginosa were evaluated based on coculture experiments with nontoxic Dolichospermum (Anabaena) flos-aquae. The MC-LR quota and transcription of mcyB and mcyD genes encoding MC synthetases in M. aeruginosa were evaluated on the basis of cell counts
high-performance liquid chromatography
and reverse-transcription quantitative real-time PCR. The MC-LR quotas of M. aeruginosa in coculture with a 1/1 ratio of inoculum of the two species were significantly lower relative to monocultures 6-d after inoculation. Decreased MC-LR quotas under coculture conditions were enhanced by increasing the D. flos-aquae to M. aeruginosa ratio in the inoculum and by environmental factors
such as temperature and light intensity. Moreover
the transcriptional concentrations of mcyB and mcyD genes in M. aeruginosa were significantly inhibited by D. flos-aquae competition in coculture (P <0.01)
lowered to 20% of initial concentrations within 8 days. These data suggested that coculture eff ects by D. flos-aquae not only reduced M. aeruginosa’s intracellular MC-LR quota via inhibition of genes encoding MC synthetases
but also that this eff ect was regulated by environmental factors
including temperature and light intensities.
关键词
Keywords
references
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