Scanning electron microscopy coupled with energy-dispersive X-ray spectrometry for quick detection of sulfur-oxidizing bacteria in environmental water samples

Chengjun Sun; Fenghua Jiang; Wei Gao; Xiaoyun Li; Yanzhen Yu; Xiaofei Yin; Yong Wang; Haibing Ding

doi:10.1007/s00343-016-5175-1

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Scanning electron microscopy coupled with energy-dispersive X-ray spectrometry for quick detection of sulfur-oxidizing bacteria in environmental water samples

Updated：2023-05-16

- Scanning electron microscopy coupled with energy-dispersive X-ray spectrometry for quick detection of sulfur-oxidizing bacteria in environmental water samples
- Journal of Oceanology and Limnology Vol. 35, Issue 1, Pages: 185-191(2017)
- Affiliations：
  
  1. Marine Ecology Center First Institute of Oceanography State Oceanic Administration
  2. College of Chemistry and Chemical Engineering Ocean University of China
  3. Qingdao Hao Ao Environmental Engineering Ltd.
  4. Key Laboratory of Marine Chemistry Theory and Technology Ministry of Education Ocean University of China
  5. Qingdao Collaborative Innovation Center of Marine Science and Technology Ocean University of China
- Author bio：
- Funds：
- DOI：10.1007/s00343-016-5175-1
  CLC：
- Online First：08 March 2016，
  
  Published：2017
- Accepted：
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Sun, C., Jiang, F., Gao, W. et al. Scanning electron microscopy coupled with energy-dispersive X-ray spectrometry for quick detection of sulfur-oxidizing bacteria in environmental water samples., Chin. J. Ocean. Limnol. 35, 185–191 (2017). https://doi.org/10.1007/s00343-016-5175-1
DOI：

Sun, C., Jiang, F., Gao, W. et al. Scanning electron microscopy coupled with energy-dispersive X-ray spectrometry for quick detection of sulfur-oxidizing bacteria in environmental water samples., Chin. J. Ocean. Limnol. 35, 185–191 (2017). https://doi.org/10.1007/s00343-016-5175-1 DOI：

摘要

Abstract

Detection of sulfur-oxidizing bacteria has largely been dependent on targeted gene sequencing technology or traditional cell cultivation

which usually takes from days to months to carry out. This clearly does not meet the requirements of analysis for time-sensitive samples and/or complicated environmental samples. Since energy-dispersive X-ray spectrometry (EDS) can be used to simultaneously detect multiple elements in a sample

including sulfur

with minimal sample treatment

this technology was applied to detect sulfur-oxidizing bacteria using their high sulfur content within the cell. This article describes the application of scanning electron microscopy imaging coupled with EDS mapping for quick detection of sulfur oxidizers in contaminated environmental water samples

with minimal sample handling. Scanning electron microscopy imaging revealed the existence of dense granules within the bacterial cells

while EDS identified large amounts of sulfur within them. EDS mapping localized the sulfur to these granules. Subsequent 16S rRNA gene sequencing showed that the bacteria detected in our samples belonged to the genus Chromatium

which are sulfur oxidizers. Thus

EDS mapping made it possible to identify sulfur oxidizers in environmental samples based on localized sulfur within their cells

within a short time (within 24 h of sampling). This technique has wide ranging applications for detection of sulfur bacteria in environmental water samples.

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references

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