Functional characterization of a novel microbial esterase identified from the Indian Ocean and its use in the stereoselective preparation of (R)-methyl mandelate

Jiayuan Liang; Aijun Sun; Yun Zhang; Dun Deng; Yongfei Wang; Sanmei Ma; Yunfeng Hu

doi:10.1007/s00343-016-5164-4

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Functional characterization of a novel microbial esterase identified from the Indian Ocean and its use in the stereoselective preparation of (R)-methyl mandelate

Updated：2023-05-16

- Functional characterization of a novel microbial esterase identified from the Indian Ocean and its use in the stereoselective preparation of (R)-methyl mandelate
- Journal of Oceanology and Limnology Vol. 34, Issue 6, Pages: 1269-1277(2016)
- Affiliations：
  
  1. Guangdong Key Laboratory of Marine Materia Medica South China Sea Institute of Oceanology Chinese Academy of Sciences
  2. Key Laboratory of Tropical Marine Bio-Resources and Ecology South China Sea Institute of Oceanology Chinese Academy of Sciences
  3. Department of Biotechnology Jinan University
  4. South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center
- Author bio：
- Funds：
- DOI：10.1007/s00343-016-5164-4
  CLC：
- Online First：19 March 2016，
  
  Published：2016
- Accepted：
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Liang, J., Sun, A., Zhang, Y. et al. Functional characterization of a novel microbial esterase identified from the Indian Ocean and its use in the stereoselective preparation of (R)-methyl mandelate., Chin. J. Ocean. Limnol. 34, 1269–1277 (2016). https://doi.org/10.1007/s00343-016-5164-4
DOI：

Liang, J., Sun, A., Zhang, Y. et al. Functional characterization of a novel microbial esterase identified from the Indian Ocean and its use in the stereoselective preparation of (R)-methyl mandelate., Chin. J. Ocean. Limnol. 34, 1269–1277 (2016). https://doi.org/10.1007/s00343-016-5164-4 DOI：

摘要

Abstract

Genomic mining has identified a novel microbial alkaline esterase from the Indian Ocean. This esterase was overexpressed in E. coli BL21 (DE3) and further functionally characterized. Under optimal conditions (10 mmol/L substrate

pH 6.0

2 h at 40 °C)

this esterase can hydrolyze racemic methyl mandelate to (R)-methyl mandelate with very high optical purity (e.e. >99%) and yield (nearly 90%). Interestingly

the stereoselectivity of this esterase is opposite to that of two previously reported lipases that can generate (S)-methyl mandelate through the hydrolysis of racemic methyl mandelate. No organic solvents or other additives were required to optimize the optical purity and production of the final chiral product (R)-methyl mandelate

which can potentially simplify the production procedure of (R)-methyl mandelate catalyzed by esterase.

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Keywords

references

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Related Institution

Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences

Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences

Department of Biotechnology, Jinan University

Affiliated Hospital of Shandong University of Traditional Chinese Medicine

South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center

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