Experimental evaluation of fluorescent (alizarin red S and calcein) and clip-tag markers for stock assessment of ark shell, Anadara broughtonii

Shanshan Zhou; Xiumei Zhang; Wentao Li; Long Li; Xingyuan Cai

doi:10.1007/s00343-016-5137-7

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Experimental evaluation of fluorescent (alizarin red S and calcein) and clip-tag markers for stock assessment of ark shell, Anadara broughtonii

Updated：2023-05-16

- Experimental evaluation of fluorescent (alizarin red S and calcein) and clip-tag markers for stock assessment of ark shell, Anadara broughtonii
- Journal of Oceanology and Limnology Vol. 35, Issue 2, Pages: 265-274(2017)
- Affiliations：
  
  Key Laboratory of Mariculture Ministry of Education Ocean University of China
- Author bio：
- Funds：
- DOI：10.1007/s00343-016-5137-7
  CLC：
- Online First：26 April 2016，
  
  Published：2017
- Accepted：
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Zhou, S., Zhang, X., Li, W. et al. Experimental evaluation of fluorescent (alizarin red S and calcein) and clip-tag markers for stock assessment of ark shell, Anadara broughtonii, ., Chin. J. Ocean. Limnol. 35, 265–274 (2017). https://doi.org/10.1007/s00343-016-5137-7
DOI：

Zhou, S., Zhang, X., Li, W. et al. Experimental evaluation of fluorescent (alizarin red S and calcein) and clip-tag markers for stock assessment of ark shell, Anadara broughtonii, ., Chin. J. Ocean. Limnol. 35, 265–274 (2017). https://doi.org/10.1007/s00343-016-5137-7 DOI：

摘要

Abstract

Release programs to enhance stocks of ark shell (Anadara broughtonii) have been undertaken in a number of Asian countries

but their effectiveness has rarely been investigated owing to a lack of marking methods. The quality and longevity of fluorescent markers

alizarin red S (ARS) and calcein (CAL) (200 and 300 mg/L)

as well as clip tags

were tested on juvenile A. broughtonii. No significant differences in survival or shell growth were observed in juveniles stained with either of the two fluorochromes after a 160-day culture period

but the retention rate was 100% after 1 year. Fluorescent marks (≥grade 3) were observable microscopically in juveniles stained with the two fluorochromes

and some fluorescent marks (≥grade 4) were visible with the naked eye after 1 year. ARS-marked shells were brighter than those marked with CAL

and shells marked with 300 mg/L of the fluorochromes were easier to detect than those marked with 200 mg/L. Clip tags were incorporated into the shell as the bivalve grew

and the retention rate was 64.25% after 160 days. Significant differences in survival (at 30 days)

shell length (at 60

120

and 160 days)

and wet weight (at 90

120

and 160 days) were observed between the clip-tagged and control groups (all P< 0.05)

indicating that the tags may have passive effects on the ark shell. The results suggest that both ARS and CAL are suitable to mark A. broughtonii for large-scale restocking programs

and that optimal marking quality was achieved with 300 mg/L ARS. Lighter and smaller clip tags need to be developed to reduce injury and increase survival rate of clams.

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references

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