Characterization and analysis of ribosomal proteins in two marine calanoid copepods

Feifei Yang; Donghui Xu; Yunyun Zhuang; Yousong Huang; Xiaoyan Yi; Hongju Chen; Guangxing Liu; Huan Zhang

doi:10.1007/s00343-016-5129-7

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Characterization and analysis of ribosomal proteins in two marine calanoid copepods

Updated：2023-05-16

- Characterization and analysis of ribosomal proteins in two marine calanoid copepods
- Journal of Oceanology and Limnology Vol. 34, Issue 6, Pages: 1258-1268(2016)
- Affiliations：
  
  1. Key Laboratory of Marine Environment and Ecology (Ocean University of China) Ministry of Education
  2. College of Environmental Science and Engineering Ocean University of China
  3. Department of Marine Sciences University of Connecticut
- Author bio：
- Funds：
- DOI：10.1007/s00343-016-5129-7
  CLC：
- Online First：05 February 2016，
  
  Published：2016
- Accepted：
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Yang, F., Xu, D., Zhuang, Y. et al. Characterization and analysis of ribosomal proteins in two marine calanoid copepods., Chin. J. Ocean. Limnol. 34, 1258–1268 (2016). https://doi.org/10.1007/s00343-016-5129-7
DOI：

Yang, F., Xu, D., Zhuang, Y. et al. Characterization and analysis of ribosomal proteins in two marine calanoid copepods., Chin. J. Ocean. Limnol. 34, 1258–1268 (2016). https://doi.org/10.1007/s00343-016-5129-7 DOI：

摘要

Abstract

Copepods are among the most abundant and successful metazoans in the marine ecosystem. However

genomic resources related to fundamental cellular processes are still limited in this particular group of crustaceans. Ribosomal proteins are the building blocks of ribosomes

the primary site for protein synthesis. In this study

we characterized and analyzed the cDNAs of cytoplasmic ribosomal proteins (cRPs) of two calanoid copepods

Pseudodiaptomus poplesia and Acartia pacifica. We obtained 79 cRP cDNAs from P. poplesia and 67 from A. pacifica by cDNA library construction/sequencing and rapid amplification of cDNA ends. Analysis of the nucleic acid composition showed that the copepod cRP-encoding genes had higher GC content in the protein-coding regions (CDSs) than in the untranslated regions (UTRs)

and single nucleotide repeats (>3 repeats) were common

with “A” repeats being the most frequent

especially in the CDSs. The 3′-UTRs of the cRP genes were significantly longer than the 5′-UTRs. Codon usage analysis showed that the third positions of the codons were dominated by C or G. The deduced amino acid sequences of the cRPs contained high proportions of positively charged residues and had high pI values. This is the first report of a complete set of cRP-encoding genes from copepods. Our results shed light on the characteristics of cRPs in copepods

and provide fundamental data for further studies of protein synthesis in copepods. The copepod cRP information revealed in this study indicates that additional comparisons and analysis should be performed on different taxonomic categories such as orders and families.

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