Immunogenicity and protective role of antigenic regions from five outer membrane proteins of Flavobacterium columnare in grass carp Ctenopharyngodon idella

Zhang Luo; Zhixin Liu; Jianping Fu; Qiusheng Zhang; Bei Huang; Pin Nie

doi:10.1007/s00343-016-5096-z

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Immunogenicity and protective role of antigenic regions from five outer membrane proteins of Flavobacterium columnare in grass carp Ctenopharyngodon idella

Updated：2023-05-16

- Immunogenicity and protective role of antigenic regions from five outer membrane proteins of Flavobacterium columnare in grass carp Ctenopharyngodon idella
- Journal of Oceanology and Limnology Vol. 34, Issue 6, Pages: 1247-1257(2016)
- Affiliations：
  
  1. College of Fisheries Huazhong Agricultural University
  2. State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences
  3. Tianjin Fishery Research Institute
  4. College of Fisheries Jimei University
- Author bio：
- Funds：
- DOI：10.1007/s00343-016-5096-z
  CLC：
- Online First：05 February 2016，
  
  Published：2016
- Accepted：
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Luo, Z., Liu, Z., Fu, J. et al. Immunogenicity and protective role of antigenic regions from five outer membrane proteins of Flavobacterium columnare in grass carp Ctenopharyngodon idella, ., Chin. J. Ocean. Limnol. 34, 1247–1257 (2016). https://doi.org/10.1007/s00343-016-5096-z
DOI：

Luo, Z., Liu, Z., Fu, J. et al. Immunogenicity and protective role of antigenic regions from five outer membrane proteins of Flavobacterium columnare in grass carp Ctenopharyngodon idella, ., Chin. J. Ocean. Limnol. 34, 1247–1257 (2016). https://doi.org/10.1007/s00343-016-5096-z DOI：

摘要

Abstract

Flavobacterium columnare causes columnaris disease in freshwater fish. In the present study

the antigenic regions of five outer membrane proteins (OMPs)

including zinc metalloprotease

prolyl oligopeptidase

thermolysin

collagenase and chondroitin AC lyase

were bioinformatically analyzed

fused together

and then expressed as a recombinant fusion protein in Escherichia coli. The expressed protein of 95.6 kDa

as estimated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis

was consistent with the molecular weight deduced from the amino acid sequence. The purified recombinant protein was used to vaccinate the grass carp

Ctenopharyngodon idella. Following vaccination of the fish their IgM antibody levels were examined

as was the expression of IgM

IgD and IgZ immunoglobulin genes and other genes such as MHC Iα and MHC IIβ

which are also involved in adaptive immunity. Interleukin genes (IL)

including IL-1β

IL-8 and IL-10

and type I and type II interferon (IFN) genes were also examined. At 3 and 4 weeks post-vaccination (wpv)

significant increases in IgM antibody levels were observed in the fish vaccinated with the recombinant fusion protein

and an increase in the expression levels of IgM

IgD and IgZ genes was also detected following the vaccinations

thus indicating that an adaptive immune response was induced by the vaccinations. Early increases in the expression levels of IL and IFN genes were also observed in the vaccinated fish. At four wpv

the fish were challenged with F. columnare

and the vaccinated fish showed a good level of protection against this pathogen

with 39% relative percent survival (RPS) compared with the control group. It can be concluded

therefore

that the five OMPs

in the form of a recombinant fusion protein vaccine

induced an immune response in fish and protection against F. columnare.

关键词

Keywords

references

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