Establishment and characterization of a testicular Sertoli cell line from olive flounder Paralichthys olivaceus

Limin Peng; Yuan Zheng; Feng You; Zhihao Wu; Yuxia Zou; Peijun Zhang

doi:10.1007/s00343-016-5091-4

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Establishment and characterization of a testicular Sertoli cell line from olive flounder Paralichthys olivaceus

Updated：2023-05-16

- Establishment and characterization of a testicular Sertoli cell line from olive flounder Paralichthys olivaceus
- Journal of Oceanology and Limnology Vol. 34, Issue 5, Pages: 1054-1063(2016)
- Affiliations：
  
  1. Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences Qingdao National Laboratory for Marine Science and Technology
  2. Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology
  3. University of Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-016-5091-4
  CLC：
- Online First：24 November 2015，
  
  Published：2016
- Accepted：
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Peng, L., Zheng, Y., You, F. et al. Establishment and characterization of a testicular Sertoli cell line from olive flounder Paralichthys olivaceus, ., Chin. J. Ocean. Limnol. 34, 1054–1063 (2016). https://doi.org/10.1007/s00343-016-5091-4
DOI：

Peng, L., Zheng, Y., You, F. et al. Establishment and characterization of a testicular Sertoli cell line from olive flounder Paralichthys olivaceus, ., Chin. J. Ocean. Limnol. 34, 1054–1063 (2016). https://doi.org/10.1007/s00343-016-5091-4 DOI：

摘要

Abstract

The culture of Sertoli cells has become an indispensable resource in studying spermatogenesis. A new Sertoli cell line (POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages. Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P. olivaceus. Cells were optimally maintained at 25°C in DMEM/F12 medium supplemented with fetal bovine serum

basic fibroblast growth factor

and epidermal growth factor. The growth curve of POSC showed a typical “S” shape. Chromosome analysis revealed that the cell line possessed the normal P. olivaceus diploid karyotype of 2n=48t. POSC expressed dmrt1 but not vasa

which was detected using RT-PCR and sequencing. Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL. Therefore

POSC appeared to consist of testicular Sertoli cells. Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid

with the transfection efficiency reaching 10%. This research not only offers an ideal model for further gene expression and regulation studies on P. olivaceus

but also serves as valuable material in studying fish spermatogenesis

Sertoli cell-germ cell interactions

and the mechanism of growth and development of testis.

关键词

Keywords

references

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