Quantitative trait loci detection of Edwardsiella tarda resistance in Japanese flounder Paralichthys olivaceus using bulked segregant analysis

Xiaoxia Wang; Wenteng Xu; Yang Liu; Lei Wang; Hejun Sun; Lei Wang; Songlin Chen

doi:10.1007/s00343-016-5080-7

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Quantitative trait loci detection of Edwardsiella tarda resistance in Japanese flounder Paralichthys olivaceus using bulked segregant analysis

Updated：2023-05-16

- Quantitative trait loci detection of Edwardsiella tarda resistance in Japanese flounder Paralichthys olivaceus using bulked segregant analysis
- Journal of Oceanology and Limnology Vol. 34, Issue 6, Pages: 1297-1308(2016)
- Affiliations：
  
  1. Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Function Laboratory for Marine Fisheries Science and Food Production Processes National Lab for Ocean Science and Technology
  2. College of Marine Life Science Shanghai Ocean University
  3. Nanjing Agricultural University
  4. College of Fisheries Henan Normal University
- Author bio：
- Funds：
- DOI：10.1007/s00343-016-5080-7
  CLC：
- Online First：16 March 2016，
  
  Published：2016
- Accepted：
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Wang, X., Xu, W., Liu, Y. et al. Quantitative trait loci detection of Edwardsiella tarda resistance in Japanese flounder Paralichthys olivaceus using bulked segregant analysis., Chin. J. Ocean. Limnol. 34, 1297–1308 (2016). https://doi.org/10.1007/s00343-016-5080-7
DOI：

Wang, X., Xu, W., Liu, Y. et al. Quantitative trait loci detection of Edwardsiella tarda resistance in Japanese flounder Paralichthys olivaceus using bulked segregant analysis., Chin. J. Ocean. Limnol. 34, 1297–1308 (2016). https://doi.org/10.1007/s00343-016-5080-7 DOI：

摘要

Abstract

In recent years

Edwardsiella tarda has become one of the most deadly pathogens of Japanese flounder (Paralichthys olivaceus)

causing serious annual losses in commercial production. In contrast to the rapid advances in the aquaculture of P. olivaceus

the study of E. tarda resistance-related markers has lagged behind

hindering the development of a disease-resistant strain. Thus

a marker-trait association analysis was initiated

combining bulked segregant analysis (BSA) and quantitative trait loci (QTL) mapping. Based on 180 microsatellite loci across all chromosomes

106 individuals from the F1333 (♀: F0768 ×♂: F0915) (Nomenclature rule: F+year+family number) were used to detect simple sequence repeats (SSRs) and QTLs associated with E. tarda resistance. After a genomic scan

three markers (Scaffold 404-21589

Scaffold 404-21594 and Scaffold 270-13812) from the same linkage group (LG)-1 exhibited a significant difference between DNA

pooled/bulked from the resistant and susceptible groups (P <0.001). Therefore

106 individuals were genotyped using all the SSR markers in LG1 by single marker analysis. Two different analytical models were then employed to detect SSR markers with different levels of significance in LG1

where 17 and 18 SSR markers were identified

respectively. Each model found three resistance-related QTLs by composite interval mapping (CIM). These six QTLs

designated qE1–6

explained 16.0%–89.5% of the phenotypic variance. Two of the QTLs

qE-2 and qE-4

were located at the 66.7 cM region

which was considered a major candidate region for E. tarda resistance. This study will provide valuable data for further investigations of E. tarda resistance genes and facilitate the selective breeding of disease-resistant Japanese flounder in the future.

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