Characterization and in-vivo evaluation of potential probiotics of the bacterial flora within the water column of a healthy shrimp larviculture system

Ming Xue; Huafang Liang; Yaoyao He; Chongqing Wen

doi:10.1007/s00343-016-5024-2

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Characterization and in-vivo evaluation of potential probiotics of the bacterial flora within the water column of a healthy shrimp larviculture system

Updated：2023-05-16

- Characterization and in-vivo evaluation of potential probiotics of the bacterial flora within the water column of a healthy shrimp larviculture system
- Journal of Oceanology and Limnology Vol. 34, Issue 3, Pages: 484-491(2016)
- Affiliations：
  
  Fisheries College Guangdong Ocean University
- Author bio：
- Funds：
- DOI：10.1007/s00343-016-5024-2
  CLC：
- Online First：24 November 2015，
  
  Published：2016
- Accepted：
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Xue, M., Liang, H., He, Y. et al. Characterization and in-vivo evaluation of potential probiotics of the bacterial flora within the water column of a healthy shrimp larviculture system., Chin. J. Ocean. Limnol. 34, 484–491 (2016). https://doi.org/10.1007/s00343-016-5024-2
DOI：

Xue, M., Liang, H., He, Y. et al. Characterization and in-vivo evaluation of potential probiotics of the bacterial flora within the water column of a healthy shrimp larviculture system., Chin. J. Ocean. Limnol. 34, 484–491 (2016). https://doi.org/10.1007/s00343-016-5024-2 DOI：

摘要

Abstract

A thorough understanding of the normal bacterial flora associated with shrimp larviculture systems contributes to probiotic screening and disease control. The bacterial community of the water column over a commercial Litopenaeus vannamei larval rearing run was characterized with both culture-dependent and culture-independent methods. A total of 27 phylotypes at the species level were isolated and identified based on 16S rDNA sequence analysis. Denaturing gradient gel electrophoresis (DGGE) analysis of the V3–V5 region of 16S rRNA genes showed a dynamic bacterial community with major changes occurred from stages zoea to mysis during the rearing run. The sequences retrieved were affiliated to four phyla

Proteobacteria

Actinobacteria

Bacteroidetes

and Firmicutes

with the family Rhodobacteraceae being the most frequently recovered one. Subsequently

13 representative strains conferred higher larval survival than the control when evaluated in the in-vivo experiments; in particular

three candidates

assigned to Phaeobacter sp.

Arthrobacter sp.

and Microbacterium sp.

significantly improved larval survival (P < 0.05). Therefore

the healthy shrimp larviculture system harbored a diverse and favorable bacterial flora

which contribute to larval development and are of great importance in exploiting novel probiotics.

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references

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