Construction of a cDNA library for sea cucumber Acaudina leucoprocta and differential expression of ferritin peptide

Jun Zhou; Fujing Hou; Ye Li; Xiurong Su; Taiwu Li; Chunhua Jin

doi:10.1007/s00343-016-5010-8

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Construction of a cDNA library for sea cucumber Acaudina leucoprocta and differential expression of ferritin peptide

Updated：2023-05-16

- Construction of a cDNA library for sea cucumber Acaudina leucoprocta and differential expression of ferritin peptide
- Journal of Oceanology and Limnology Vol. 34, Issue 4, Pages: 719-729(2016)
- Affiliations：
  
  1. School of Marine Sciences Ningbo University
  2. Ningbo Green-Health Pharmaceutical Co. Ltd.
  3. Ningbo City College of Vocational Technology
- Author bio：
- Funds：
- DOI：10.1007/s00343-016-5010-8
  CLC：
- Online First：28 January 2016，
  
  Published：2016
- Accepted：
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Zhou, J., Hou, F., Li, Y. et al. Construction of a cDNA library for sea cucumber Acaudina leucoprocta and differential expression of ferritin peptide., Chin. J. Ocean. Limnol. 34, 719–729 (2016). https://doi.org/10.1007/s00343-016-5010-8
DOI：

Zhou, J., Hou, F., Li, Y. et al. Construction of a cDNA library for sea cucumber Acaudina leucoprocta and differential expression of ferritin peptide., Chin. J. Ocean. Limnol. 34, 719–729 (2016). https://doi.org/10.1007/s00343-016-5010-8 DOI：

摘要

Abstract

Acaudina leucoprocta is an edible sea cucumber of economic interest that is widely distributed in China. Little information is available concerning the molecular genetics of this species although such knowledge would contribute to a better understanding of the optimal conditions for its aquaculture and its mechanisms of defense against disease. Therefore

we constructed a cDNA library and

based on bioinformatics analysis of the sequences

the functions of 75% of the cDNAs were identified

including those involved in cell structure

energy metabolism

mitochondrial function

and signal transduction pathways. Approximately 25% of genes in the library were unmatched. The gene for A. leucoprocta ferritin was also cloned. The predicted amino-acid sequence of ferritin displayed significant homology with other sea-cucumber counterparts but indicated that it was a new member of the ferritin family. Semiquantitative real-time RT-PCR indicated the highest levels of ferritin mRNA expression in the intestine. A polyclonal antibody of ferritin was also produced. These data provide a set of molecular tools essential for further studies of the functions of ferritin protein in A. leucoprocta.

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references

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