Screening and isolation of the algicidal compounds from marine green alga Ulva intestinalis

Xue Sun; Haoliang Jin; Lin Zhang; Wei Hu; Yahe Li; Nianjun Xu

doi:10.1007/s00343-016-4383-z

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Screening and isolation of the algicidal compounds from marine green alga Ulva intestinalis

Updated：2023-05-16

- Screening and isolation of the algicidal compounds from marine green alga Ulva intestinalis
- Journal of Oceanology and Limnology Vol. 34, Issue 4, Pages: 781-788(2016)
- Affiliations：
  
  Key Laboratory of Marine Biotechnology of Zhejiang Province School of Marine Sciences Ningbo University
- Author bio：
- Funds：
- DOI：10.1007/s00343-016-4383-z
  CLC：
- Online First：16 July 2015，
  
  Published：2016
- Accepted：
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Sun, X., Jin, H., Zhang, L. et al. Screening and isolation of the algicidal compounds from marine green alga Ulva intestinalis, ., Chin. J. Ocean. Limnol. 34, 781–788 (2016). https://doi.org/10.1007/s00343-016-4383-z
DOI：

Sun, X., Jin, H., Zhang, L. et al. Screening and isolation of the algicidal compounds from marine green alga Ulva intestinalis, ., Chin. J. Ocean. Limnol. 34, 781–788 (2016). https://doi.org/10.1007/s00343-016-4383-z DOI：

摘要

Abstract

Twenty species of seaweed were collected from the coast of Zhejiang

China

extracted with ethanol

and screened for algicidal activity against red tide microalgae Heterosigma akashiwo and Prorocentrum micans. Inhibitory effects of fresh and dried tißsues of green alga Ulva intestinalis were assessed and the main algicidal compounds were isolated

purified

and identified. Five seaweed species

U. intestinalis

U. fasciata

Grateloupia romosissima

Chondria crassicaulis

and Gracilariopsis lemaneiformis

were investigated for their algicidal activities. Fresh tissues of 8.0 and 16.0 mg/mL of U. intestinalis dissolved in media significantly inhibited growth of H. akashiwo and P. micans

respectively. Dried tissue and ethyl acetate (EtOAc) extracts of U. intestinalis at greater than 1.2 and 0.04 mg/mL

respectively

were fatal to H. akashiwo

while its water and EtOAc extracts in excess of 0.96 and 0.32 mg/mL

respectively

were lethal to P. micans. Three algicidal compounds in the EtOAc extracts were identified as 15-ethoxy-(6z

12z)-hexadecatrienoic acid (I)

(6E

12E)-(2-acetoxy-β-D-glucose)-octadecatrienoic acid ester (II) and hexadecanoic acid (III). Of these

compound II displayed the most potent algicidal activity with IC50 values of 4.9 and 14.1 µg/mL for H. akashiwo and P. micans

respectively. Compound I showed moderate algicidal activity with IC50 values of 13.4 and 24.7 µg/mL for H. akashiwo and P. micans

respectively. These findings suggested that certain macroalgae or products therefrom could be used as effective biological control agents against red tide algae.

关键词

Keywords

references

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