Production of γ-linolenic acid and stearidonic acid by Synechococcus sp. PCC7002 containing cyanobacterial fatty acid desaturase genes

Xuewei Dong; Qingfang He; Zhenying Peng; Jinhui Yu; Fei Bian; Youzhi Li; Yuping Bi

doi:10.1007/s00343-016-4369-x

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Production of γ-linolenic acid and stearidonic acid by Synechococcus sp. PCC7002 containing cyanobacterial fatty acid desaturase genes

Updated：2023-05-16

- Production of γ-linolenic acid and stearidonic acid by Synechococcus sp. PCC7002 containing cyanobacterial fatty acid desaturase genes
- Journal of Oceanology and Limnology Vol. 34, Issue 4, Pages: 772-780(2016)
- Affiliations：
  
  1. College of Life Science and Technology State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangxi University
  2. Biotechnology Research Center Shandong Academy of Agricultural Science Key Laboratory for Genetic Improvement of Crop Animal and Poultry of Shandong Province Key Laboratory of Crop Genetic Improvement and Biotechnology Huanghuaihai Ministry of Agriculture
  3. Department of Applied Science University of Arkansas
- Author bio：
- Funds：
- DOI：10.1007/s00343-016-4369-x
  CLC：
- Online First：24 November 2015，
  
  Published：2016
- Accepted：
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Dong, X., He, Q., Peng, Z. et al. Production of γ-linolenic acid and stearidonic acid by Synechococcus sp. PCC7002 containing cyanobacterial fatty acid desaturase genes., Chin. J. Ocean. Limnol. 34, 772–780 (2016). https://doi.org/10.1007/s00343-016-4369-x
DOI：

Dong, X., He, Q., Peng, Z. et al. Production of γ-linolenic acid and stearidonic acid by Synechococcus sp. PCC7002 containing cyanobacterial fatty acid desaturase genes., Chin. J. Ocean. Limnol. 34, 772–780 (2016). https://doi.org/10.1007/s00343-016-4369-x DOI：

摘要

Abstract

Genetic modification is useful for improving the nutritional qualities of cyanobacteria. To increase the total unsaturated fatty acid content

along with the ratio of ω-3/ω-6 fatty acids

genetic engineering can be used to modify fatty acid metabolism. Synechococcus sp. PCC7002

a fast-growing cyanobacterium

does not contain a Δ6 desaturase gene and is therefore unable to synthesize γ-linolenic acid (GLA) and stearidonic acid (SDA)

which are important in human health. In this work

we constructed recombinant vectors Syd6D

Syd15D and Syd6Dd15D to express the Δ15 desaturase and Δ6 desaturase genes from Synechocystis PCC6803 in Synechococcus sp. PCC7002

with the aim of expressing polyunsaturated fatty acids. Overexpression of the Δ15 desaturase gene in Synechococcus resulted in 5.4 times greater accumulation of α-linolenic acid compared with the wild-type while Δ6 desaturase gene expression produced both GLA and SDA. Co-expression of the two genes resulted in low-level accumulation of GLA but much larger amounts of SDA

accounting for as much to 11.64% of the total fatty acid content.

关键词

Keywords

references

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