Identification and characterization of a DnaJ gene from red alga Pyropia yezoensis (Bangiales, Rhodophyta)

Jiao Liu; Xianchao Li; Xuexi Tang; Bin Zhou

doi:10.1007/s00343-015-4321-5

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Identification and characterization of a DnaJ gene from red alga Pyropia yezoensis (Bangiales, Rhodophyta)

Updated：2023-05-16

- Identification and characterization of a DnaJ gene from red alga Pyropia yezoensis (Bangiales, Rhodophyta)
- Journal of Oceanology and Limnology Vol. 34, Issue 2, Pages: 405-411(2016)
- Affiliations：
  
  College of Marine Life Science Ocean University of China
- Author bio：
- Funds：
- DOI：10.1007/s00343-015-4321-5
  CLC：
- Online First：01 July 2015，
  
  Published：2016
- Accepted：
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Liu, J., Li, X., Tang, X. et al. Identification and characterization of a DnaJ gene from red alga Pyropia yezoensis (Bangiales, Rhodophyta)., Chin. J. Ocean. Limnol. 34, 405–411 (2016). https://doi.org/10.1007/s00343-015-4321-5
DOI：

Liu, J., Li, X., Tang, X. et al. Identification and characterization of a DnaJ gene from red alga Pyropia yezoensis (Bangiales, Rhodophyta)., Chin. J. Ocean. Limnol. 34, 405–411 (2016). https://doi.org/10.1007/s00343-015-4321-5 DOI：

摘要

Abstract

Members of the DnaJ family are proteins that play a pivotal role in various cellular processes

such as protein folding

protein transport and cellular responses to stress. In the present study

we identified and characterized the full-length DnaJ cDNA sequence from expressed sequence tags of Pyropia yezoensis (PyDnaJ) via rapid identification of cDNA ends. This cDNA encoded a protein of 429 amino acids

which shared high sequence similarity with other identified DnaJ proteins

such as a heat shock protein 40/DnaJ from Pyropia haitanensis. The relative mRNA expression level of PyDnaJ was investigated using real-time PCR to determine its specific expression during the algal life cycle and during desiccation. The relative mRNA expression level in sporophytes was higher than that in gametophytes and significantly increased during the whole desiccation process. These results indicate that PyDnaJ is an authentic member of the DnaJ family in plants and red algae and might play a pivotal role in mitigating damage to P. yezoensis during desiccation.

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references

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