Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica

Wenjing Zhang; Delin Sun; Xia Zhao; Weihua Jin; Jing Wang; Quanbin Zhang

doi:10.1007/s00343-015-4273-9

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Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica

Updated：2023-05-16

- Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica
- Journal of Oceanology and Limnology Vol. 34, Issue 1, Pages: 177-185(2016)
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
  3. Heze Junxinyuan Food Company
  4. Ocean University of China
- Author bio：
- Funds：
- DOI：10.1007/s00343-015-4273-9
  CLC：
- Online First：17 June 2015，
  
  Published：2016
- Accepted：
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Zhang, W., Sun, D., Zhao, X. et al. Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica, ., Chin. J. Ocean. Limnol. 34, 177–185 (2016). https://doi.org/10.1007/s00343-015-4273-9
DOI：

Zhang, W., Sun, D., Zhao, X. et al. Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica, ., Chin. J. Ocean. Limnol. 34, 177–185 (2016). https://doi.org/10.1007/s00343-015-4273-9 DOI：

摘要

Abstract

A rapid

sensitive and reproducible high performance liquid chromatography (HPLC) method with post-column fluorescence derivatization has been developed to determine the amount of low-molecular-weight sulfated polysaccharide (GFS) in vivo. The metabolism of GFS has been shown to fit a two component model following its administration by intravenous injection

and its pharmacokinetic parameters were determined to be as follows: half-time of distribution phase (t 1/2α)=11.24±2.93 min

half-time of elimination phase (t 1/2β)=98.20±25.78 min

maximum concentration (C max)=110.53 μg/mL and peak time (T max)=5 min. The pharmacokinetic behavior of GFS was also investigated following intragastric administration. However

the concentration of GFS found in serum was too low for detection

and GFS could only be detected for up to 2 h after intragastric administration (200 mg/kg body weight). Thus

the bioavailability of GFS was low following intragastric administration because of the metabolism of GFS. In conclusion

HPLC with postcolumn derivatization could be used for quantitative microanalysis and pharmacokinetic studies to determine the presence of polysaccharides in the serum following intravenous injection.

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references

Han Y H, Lv Z H, Jiang T F, Wang Y H. 2007. Bioanalysis and pharmacokinetics of chitosan ester in rabbit serum by HPLC with postcolumn fluorescence derivatization. Journal of Chromatography B, 845 (1): 138–142.

Honda S. 1996. Postcolumn derivatization for chromatographic analysis of carbohydrates. Journal of Chromatography A, 720 (1-2): 183–199.

Huang Y, Washio Y, Hara M, Toyoda H, Koshiishi I, Toida T, Imanari T. 1996. Simultaneous determination of dermatan sulfate and oversulfated dermatan sulfate in plasma by high-performance liquid chromatography with postcolumn fluorescence derivatization. Analytical Biochemistry, 240 (2): 227–234.

Jin W H, Wang J, Ren S M, Song N, Zhang Q B. 2012. Structural analysis of a heteropolysaccharide from Saccharina japonica by electrospray mass spectrometry in tandem with collision-induced dissociation tandem mass spectrometry (ESI-CID-MS/MS). Marine Drugs, 10 (10): 2 138–2 152.

Jin W H, Zhang W J, Wang J, Ren S M, Song N, Zhang Q B. 2013. Structural analysis of heteropolysaccharide from Saccharina japonica and its derived oligosaccharides. International Journal of Biological Macromolecules, 62: 697–704.

Millet J, Jouault S C, Mauray S, Theveniaux J, Sternberg C, Boisson Vidal C, Fischer A M. 1999. Antithrombotic and anticoagulant activities of a low molecular weight fucoidan by the subcutaneous route. Thrombosis and Haemostasis, 81 (3): 391–395.

Mizuno M, Nishitani Y, Tanoue T, Matoba Y, Ojima T, Hashimoto T, Kanazawa K. 2009. Quantification and localization of fucoidan in Laminaria japonica using a novel antibody. Bioscience, Biotechnology, and Biochemistry, 73 (2): 335–338.

Olsson Y, Svensjö E, Arfors K E, Hultström D. 1975. Fluorescein labelled dextrans as tracers for vascular permeability studies in the nervous system. Acta Neuropathologica, 33 (1): 45–50.

Polson C, Sarkar P, Incledon B, Raguvaran V, Grant R. 2003. Optimization of protein precipitation based upon effectiveness of protein removal and ionization effect in liquid chromatography-tandem mass spectrometry. Journal of Chromatography B, 785 (2): 263–275.

Sun S M, Zhao X, Li G S, Yu G L, Xing X X, Zeng Y Y, Wu J, Wang J N. 2012. Microanalysis of oligosaccharide HS203 in beagle dog plasma by postcolumn fluorescence derivatization method. Carbohydrate Polymers, 89 (2): 661–666.

Suzuki S, Fujimori T, Yodoshi M. 2006. Recovery of free oligosaccharides from derivatives labeled by reductive amination. Analytical Biochemistry, 354 (1): 94–103.

Toida T, Huang Y, Washio Y, Maruyama T, Toyoda H, Imanari T, Linhardt R J. 1997. Chemical microdetermination of heparin in plasma. Analytical Biochemistry, 251 (2): 219–226.

Tokita Y, Nakajima K, Mochida H, Iha M, Nagamine T. 2010. Development of a fucoidan-specific antibody and measurement of fucoidan in serum and urine by sandwich ELISA. Bioscience, Biotechnology, and Biochemistry, 74 (2): 350–357.

Trento F, Cattaneo F, Pescador R, Porta R, Ferro L. 2001. Antithrombin activity of an algal polysaccharide. Thrombosis Research, 102 (5): 457–465.

Wang J, Wang F, Hou Y, Zhang H, Zhang Q B. 2012. Effect and mechanism of fucoidan derivatives from Laminaria japonica in experimental adenine-induced chronic kidney disease. Journal of Ethnopharmacology, 139 (3): 807–813.

Wang J, Zhang Q B, Zhang Z S, Li Z E. 2008. Antioxidant activity of sulfated polysaccharide fractions extracted from Laminaria japonica. International Journal of Biological Macromolecules, 42 (2): 127–132.

Wang J, Zhang Q B, Zhang Z S, Song H F, Li P C. 2010a. Potential antioxidant and anticoagulant capacity of low molecular weight fucoidan fractions extracted from Laminaria japonica. International Journal of Biological Macromolecules, 46 (1): 6–12.

Wang W T, Zhou J H, Xing S T, Guan H S. 1994. Immunomodulating action of marine algae sulfated polysaccharides on normal and immunosuppressed mice. Chinese Journal of Pharmacology and Toxicology, 8 (3): 199–199. (in Chinese with English abstract)

Wang Y, Yin H P, Lv X B, Wang Y F, Gao H Y, Wang M. 2010b. Protection of chronic renal failure by a polysaccharide from Cordyceps sinensis. Fitoterapia, 81 (5): 397–402.

Wijesekara I, Pangestuti R, Kim S K. 2011. Biological activities and potential health benefits of sulfated polysaccharides derived from marine algae. Carbohydrate Polymers, 84 (1): 14–21.

Zhang J J, Zhang Q B, Wang J, Shi X L, Zhang Z S. 2009. Analysis of the monosaccharide composition of fucoidan by precolumn derivation HPLC. Chinese Journal of Oceanology and Limnology, 27 (3): 578–582. (in Chinese with English abstract)

Zhang W J, Wang J, Jin W H, Zhang Q B. 2013. The antioxidant activities and neuroprotective effect of polysaccharides from the starfish Asterias rollestoni. Carbohydrate Polymers, 95 (1): 9–15.

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