Expression responses of five cold tolerant related genes to two temperature dropping treatments in sea cucumber Apostichopus japonicus

Chengze Li; Yaqing Chang; Zhenguo Pang; Jun Ding; Nanjing Ji

doi:10.1007/s00343-015-4148-0

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Expression responses of five cold tolerant related genes to two temperature dropping treatments in sea cucumber Apostichopus japonicus

Updated：2023-05-16

- Expression responses of five cold tolerant related genes to two temperature dropping treatments in sea cucumber Apostichopus japonicus
- Journal of Oceanology and Limnology Vol. 33, Issue 2, Pages: 309-318(2015)
- Affiliations：
  
  Key Laboratory of Mariculture and Stock Enhancement in North China’s Sea Ministry of Agriculture Dalian Ocean University
- Author bio：
- Funds：
- DOI：10.1007/s00343-015-4148-0
  CLC：
- Online First：10 December 2014，
  
  Published：2015
- Accepted：
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Li, C., Chang, Y., Pang, Z. et al. Expression responses of five cold tolerant related genes to two temperature dropping treatments in sea cucumber Apostichopus japonicus, ., Chin. J. Ocean. Limnol. 33, 309–318 (2015). https://doi.org/10.1007/s00343-015-4148-0
DOI：

Li, C., Chang, Y., Pang, Z. et al. Expression responses of five cold tolerant related genes to two temperature dropping treatments in sea cucumber Apostichopus japonicus, ., Chin. J. Ocean. Limnol. 33, 309–318 (2015). https://doi.org/10.1007/s00343-015-4148-0 DOI：

摘要

Abstract

Environmental conditions

including ambient temperature

play important roles in survival

growth development

and reproduction of the Japanese sea cucumber

Apostichopus japonicus. Low temperatures result in slowed growth and skin ulceration disease. In a previous study

we investigated the effect of low temperature on gene expression profiles in A. japonicus by suppression subtractive hybridization (SSH). Genes encoding Ferritin

Lysozyme

Hsp70

gp96

and AjToll were selected from a subtracted cDNA library of A. japonicus under acute cold stress. The transcriptional expression profiles of these genes were investigated in different tissues (coelomocyte

respiratory tree

intestine

longitudinal muscle) after exposure to acute and mild temperature dropping treatments. The results show that (1) the five cold-tolerance-related genes were found in all four tissues and the highest mRNA levels were observed in coelomocyte and respiratory tree; (2) under the temperature dropping treatments

three types of transcriptional regulation patterns were observed: primary suppression followed by up-regulation at −2°C

suppressed expression throughout the two treatments

and more rarely an initial stimulation followed by suppression; and (3) gene expression suppression was more severe under acute temperature dropping than under mild temperature dropping treatment. The five cold-tolerance-related genes that were distributed mainly in coelomocyte and respiratory tissues were generally down-regulated by low temperature stress but an inverse up-regulation event was found at the extreme temperature (−2°C).

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references

Ali K S, Dorgai L, Abrahám M, Hermesz E. 2003. Tissue- and stressor-specific differential expression of two hsc70 genes in carp. Biochemical and Biophysical Research Communications, 307: 503–509.

Beck G, Ellis T W, Habicht G S, Schluter S F, Marchalonis J J. 2002. Evolution of the acute phase response: iron release by echinoderm (Asterias forbesi) coelomocytes, and cloning of an echinoderm ferritin molecule. Developmental and Comparative Immunology, 26: 11–26.

Chang Y Q, Shi S B, Zhao C, Han Z H. 2011. Characteristics of papillae in wild, cultivated and hybrid sea cucumbers (Apostichopus japonicus). African Journal of Biotechnology, 10: 13 780–13 788.

Chen B, Zhong D, Monteiro A. 2006. Comparative genomics and evolution of the HSP90 family of genes across all kingdoms of organisms. BMC Genomics, 7: 156–174.

Ciancio M, Chang E. 2008. Do heat shock proteins play any role in gut inflammation? Inflammatory Bowel Diseases, 14: S102–3.

Dolmatov I Y, Ginanova T T. 2009. Post-autotomy regeneration of respiratory trees in the holothurian Apostichopus japonicus (Holothuroidea, Aspidochirotida). Cell and Tissue Research, 336: 41–58.

Dong Y W, Dong S L, Meng X L. 2008. Effects of thermal and osmotic stress on growth, osmoregulation and Hsp70 in sea cucumber (Apostichopus japonicus Selenka). Aquaculture, 276: 179–186.

Dong Y W, Ji T T, Dong S L. 2007. Stress responses to rapid temperature changes of the juvenile sea cucumber (Apostichopus japonicus Selenka). Journal of Ocean University of China, 6: 275–280.

Du H, Bao Z, Hou R, Wang S, Su H, Yan J, Tian M, Li Y, Wei W, Lu W, Hu X, Wang S, Hu J. 2012. Transcriptome sequencing and characterization for the sea cucumber Apostichopus japonicus (Selenka, 1867). Plo S One, 7: 1–10.

Feder M, Hofmann G. 1999. Heat shock proteins, molecular chaperones, and the stress response: evolutionary and ecological physiology. Annual Review of Physiology, 61: 23–82.

Gao F, Yang H, Xu Q, Wang F, Liu G. 2009. Effect of water temperature on digestive enzyme activity and gut mass in sea cucumber Apostichopus japonicus (Selenka), with special reference to aestivation. Chinese Journal of Oceanology and Limnology, 27: 714–722.

Grorgi A, Mignogna G, Bellapadrona G, Gattoni M, Chiaraluce R, Consalvi V, Chiancone E, Stefanini S. 2008. The unusual co-assembly of H- and M-chains in the ferritin molecule from the Antarctic teleosts Trematomus bernacchii and Trematomus newnesi. Archives of Biochemistry and Biophysics, 478: 69–74.

Gueguen Y, Cadoret J P, Flament D, Barreau-Roumiguière C, Girardot A L, Garnier J, Hoareau A, Bachère E, Escoubas J M. 2003. Immune gene discovery by expressed sequence tags generated from hemocytes of the bacteria-challenged oyster, Crassostrea gigas. Gene, 303: 139–145.

Ji C L, Sun G H, Yang J M, Song Z L, Liu X Q, Wang W J. 2011. Construction and analysis of the subtracted cDNA library of Apostichopus Japonicus (Selenka) under high temperature stress. Oceanologia et Limnologia Sinica, 42: 60–66. (in Chinese with English abstract)

Ji T, Dong Y, Dong S. 2008. Growth and physiological responses in the sea cucumber, Apostichopus japonicus Selenka: aestivation and temperature. Aquaculture, 283: 180–187.

Johanson P T. 1969. The coelomic elements of sea urchins (Strongylocentrotus) 3. In vitro reaction to bacteria. Journal of Invertebrate Pathology, 13: 42–62.

Ju Z, Dunham R A, Liu Z. 2002. Differential gene expression in the brain of channel catfish (Ictalurus punctatus) in response to cold acclimation. Molecular Menetics and Genomics, 268: 87–95.

Kawai T, Akira S. 2010. The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors. Nature Immunology, 11: 373–384.

Kulkeaw K, Ishitani T, Kanemaru T, Ivanovski O, Nakagawa M, Mizuochi C, Horio Y, Sugiyama D. 2011. Cold exposure down-regulates zebrafish pigmentation. Genes Cells, 16: 358–367.

Li C Z, Wang X Y, Ding J, Chang Y Q. 2013. Constuction and analysis of subtracted cDNA library of sea cucumber Apostichopus japonicus under low temperature stress. Journal of Dalian Ocean University, 28: 522–527. (in Chinese with English abstract)

Liao Y L. 1980. The aspidochirote holothurians of China with erection of a new genus in echinoderms: present and past. In: Jangoux M ed. Proceedings of the European Colloquium on Echinoderms. Brussels September 1979, Rotterdam, Balkema. p.115–120.

Liu G, Yang H, Liu S. 2010. Effects of rearing temperature and density on growth, survival and development of sea cucumber larvae, Apostichopus japonicus (Selenka). Chinese Journal of Oceanology and Limnology, 28: 842–848.

Liu W, Chang Y Q, Ding J. 2013. A preliminary study of effect of mild and acute drop of temperature on serum nonspecific c immunity in ‘iyuan No.1’a cucumber populations and the cultured sea cucumber populations. Journal of Fisheries of China, 37: 1 342–1 348. (in Chinese with English abstract)

Long Y, Li L, Li Q, He X, Cui Z. 2012. Transcriptomic characterization of temperature stress responses in larval zebrafish. Plos One, 7: e37209.

Mignogna G, Chiaraluce R, Consalvi V, Cavallo S, Stefanini S, Chiancone E. 2002. Ferritin from the spleen of the Antarctic teleost Trematomus bernacchii is an M-type homopolymer. E uropean Journal of Biochemistry, 269: 1 600–1 606.

Minagawa S, Hikima J, Hirono I, Aoki T, Mori H. 2001. Expression of Japanese flounder c-type lysozyme cDNA in insect cells. Developmental and Comparative Immunology, 25: 439–445.

Moseley P. 1998. Heat shock proteins and the inflamatory response. Annals of the New York Academy of Sciences, 29: 206–213.

Nakano K, Iwama G K. 2002. The 70-kDa heat shockprotein response in two intertidal sculpins, Oligocottus maculosus and O. snyderi: relationship of hsp70 and thermal tolerance. Comparative Biochemistry and Physiology A-molecular & Integrative Physiology, 133: 79–94.

Pascoli F, Lanzano G S, Negrato E, Poltronieri C, Trocino A, Radaelli G, Bertotto D. 2011. Seasonal effects on hematological and innate immune parameters in sea bass Dicentrarchus labrax. Fish & Shellfish Immunology, 31: 1 081–1 087.

Picard D. 2002. Heat-shock protein 90: a chaperone for folding and regulation. Cellular and Molecular Life Sciences, 59: 1 640–1 648.

Ramírez-Gómez F, Aponte-Rivera F, Méndez-Castaner L, García-Arrarás J E. 2010. Changes in holothurian coelomocyte populations following immune stimulation with different molecular patterns. Fish and Shellfish Immunology, 29: 175–185.

Seppälä O, Jokela J. 2011. Immune defence under extreme ambient temperature. Biology Letters, 7: 119–122.

Sonna L A, Fujita J, Gaffi n S L, Lilly C M. 1985. Invited review: effects of heat and cold stress on mammalian gene expression. Journal of Applied Physiology, 92: 1 725–1 742.

Sun H, Zhou Z, Dong Y, Yang A, Jiang B, Gao S, Chen Z, Guan X, Wang B, Wang X. 2013. Identification and expression analysis of two Toll-like receptor genes from sea cucumber (Apostichopus japonicus). Fish and Shellfish Immunology, 34: 147–158.

Taro M, Fumiyuki G, Toshihiro Y, Toru E, Takashi S, Shinichiro K, Masamitsu S, Shigeru U. 2007. Construction of homo- and heteropolymers of plant ferritin subunits using an in vitro protein expression system. Protein Expression and Purification, 56: 237–246.

Tort L, Rotllant J, Rovira L. 1998. Immunological suppression in gilthead sea bream Sparus aurata of the North-West Mediterranean at low temperatures. Comparative Biochemistry and Physiology, Part A, 120: 175–179.

Vornanen M, Hassinen M, Koskinen H, Krasnov A. 2005. Steady-state effects of temperature acclimation on the transcriptome of the rainbow trout heart. A merican Journal of Physiology-regulatory Integrative and Comparative Physiology, 289: 1 177–1 184.

Wang F, Yang H, Gabr H R, Gao F. 2008a. Immune condition of Apostichopus japonicus during aestivation. Aquaculture, 285: 238–243.

Wang F, Yang H, Gao F, Liu G. 2008b. Effects of acute temperature or salinity stress on the immune response in sea cucumber, Apostichopus japonicus. Comparative Biochemistry and Physiology A-molecular & Integrative Physiology, 151: 491–498.

Wang P H, Liang J P, Gu Z H, Wan D H, Weng S P, Yu X Q, He J G. 2012. Molecular cloning, characterization and expression analysis of two novel Tolls (LvToll2 and LvToll3) and three putative Spatzle-like Toll ligands (LvSpz1-3) from Litopenaeus vannamei. Developmental and Comparative Immunology, 36: 359–371.

Wang X Y, Chang Y Q, Ding J, Liu W. 2012. A preliminary study of temperature reduction on immune enzymes, soluble sugars and soluble proteins in sea cucumber. Journal of Agriculture, 2: 44–49. (in Chinese with English abstract)

Xue Q, Schey K, Volety A, Chu F, La P. 2004. Purification and characterization of lysozyme from plasma of the eastern oyster (Crassostrea virginica). Comparative Biochemistry and Physiology B-biochemistry & Molecular Biology, 139: 11–25.

Yamashita M, Ojima N, Sakamoto T. 1996. Molecular cloning and cold-inducible gene expression of ferritin H subunit isoforms in rainbow trout cells. Journal of Biological Chemistry, 271: 26 908–26 913.

Yang A, Zhou Z, Dong Y, Jiang B, Wang X, Chen Z, Guan X, Wang B, Sun D. 2010. Stability comparison of cytb and β-actin genes expression in sea cucumber, Apostichopus japonicus. Journal of Agricultural Science and Techology, 12: 79–84.

Yang C, Zhang J, Li F, Ma H, Zhang Q, Jose Priya T A, Zhang X, Xiang J. 2008. A toll receptor from Chinese shrimp Fenneropenaeus chinensis is responsive to Vibrio anguillarum infection. Fish & Shellfish Immunology, 24: 564–574.

Yang H, Yuan X, Zhou Y, Mao Y, Zhang T, Liu Y. 2005. Effects of body size and water temperature on food consumption and growth in the sea cucumber Apostichopus japonicus (Selenka) with special reference to aestivation. Aquaculture Research, 36: 1 085–1 092.

Yu J H, Song J H, Choi M C, Park S W. 2009. Effects of water temperature change on immune function in surf clams, Mactra veneriformis (Bivalvia: Mactridae). Journal of Invertebrate Pathology, 102: 30–35.

Yu M Z, Chang Y Q. 2008. The effects of low temperature on some physiological phenomena in different populations of sea cucumber Apostichopus japonicas juveniles. Journal of Dalian Fisheries University, 23: 31–36. (in Chinese with English abstract)

Zhao H, Yang H S, Zhao H L, Chen M Y. 2011. Heat stressmediated gene expression in the body wall of the Japanese sea cucumber Apostichopus japonicus. Aquatic Biology, 12: 23–31.

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